Document Detail


An improved survival model of hypoxia/ischaemia in the piglet suitable for neuroprotection studies.
MedLine Citation:
PMID:  11689169     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to develop a newborn piglet model of hypoxia/ischaemia which would better emulate the clinical situation in the asphyxiated human neonate and produce a consistent degree of histopathological injury following the insult. One-day-old piglets (n=18) were anaesthetised with a mixture of propofol (10 mg/kg/h) and alfentinal (55.5 microg/kg/h) i.v. The piglets were intubated and ventilated. Physiological variables were monitored continuously. Hypoxia was induced by decreasing the inspired oxygen (FiO(2)) to 3-4% and adjusting FiO(2) to maintain the cerebral function monitor peak amplitude at < or =5 microV. The duration of the mild insult was 20 min while the severe insult was 30 min which included 10 min where the blood pressure was allowed to fall below 70% of baseline. Control piglets (n=4 of 18) were subjected to the same protocol except for the hypoxic/ischaemic insult. The piglets were allowed to recover from anaesthesia then euthanased 72 h after the insult. The brains were perfusion-fixed, removed and embedded in paraffin. Coronal sections were stained by haematoxylin/eosin. A blinded observer examined the frontal and parietal cortex, hippocampus, basal ganglia, thalamus and cerebellum for the degree of damage. The total mean histology score for the five areas of the brain for the severe insult was 15.6+/-4.4 (mean +/-S.D., n=7), whereas no damage was seen in either the mild insult (n=4) or control groups. This 'severe damage' model produces a consistent level of damage and will prove useful for examining potential neuroprotective therapies in the neonatal brain.
Authors:
K A Foster; P B Colditz; B E Lingwood; C Burke; K R Dunster; M S Roberts
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research     Volume:  919     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-11-05     Completed Date:  2002-01-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  122-31     Citation Subset:  IM    
Affiliation:
Perinatal Research Centre, University of Queensland, Royal Women's Hospital, Queensland 4029, Herston, Australia. kelley_foster@health.qld.gov.au
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn / growth & development
Blood Pressure
Brain / growth & development,  pathology
Disease Models, Animal*
Female
Heart Rate
Hypoxia-Ischemia, Brain / drug therapy,  mortality,  pathology*,  physiopathology*
Male
Monitoring, Physiologic
Neurons / pathology
Neuroprotective Agents* / therapeutic use
Survival Analysis
Swine
Chemical
Reg. No./Substance:
0/Neuroprotective Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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