Document Detail

An improved simple LC-MS/MS method for the measurement of serum aripiprazole and its major metabolite.
MedLine Citation:
PMID:  22300908     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND OBJECTIVES: Current liquid chromatographic tandem mass spectrometry (LC-MS/MS) methods to measure serum levels of aripiprazole (Ar) and dehydroaripiprazole (DHAr) are sensitive, but difficult to use in a hospital context. We aimed to develop a rapid LC-MS/MS method allowing reliable level measurement in the presence of co-administered drugs, withdrawing samples from 22 patients with acute agitation receiving 9.75 mg aripiprazole IM injection.
METHOD: We developed a sensitive and selective HPLC-MS/MS method to measure serum Ar and DHAr levels in a hospital laboratory, requiring minimal sample preparation and inferior sample volume compared to previous LC-MS/MS methods. Analytes were separated on a reversed-phase HPLC (run-time, 10 min). A triple quadrupole tandem mass spectrometer was used for quantitative analysis in positive mode by a multiple reaction monitoring. Samples were drawn 2, 4, 6, and 24h post-injection.
RESULTS: Calibration curves (2-1000 ng/mL for Ar and 3.5-500 ng/mL for DHAr) were linear, with mean correlation coefficient >0.9998. Within- and between-day precision and accuracy were within 10%. Mean recovery was 95.2 ± 4.5% for Ar and 97.6 ± 7.2% for DHAr. Ar and DHAr peaks were not affected by other co-administered psychotropic drugs.
CONCLUSION: Our method measured Ar and DHAr concentrations reliably, simply and rapidly without employing many reagents, as currently existing methods.
Matteo Caloro; Luana Lionetto; Ilaria Cuomo; Alessio Simonetti; Daniela Pucci; Simone De Persis; Barbara Casolla; Giorgio D Kotzalidis; Antonio Sciarretta; Sergio De Filippis; Maurizio Simmaco; Paolo Girardi
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Publication Detail:
Type:  Journal Article; Validation Studies     Date:  2012-01-14
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  62     ISSN:  1873-264X     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-21     Completed Date:  2012-06-25     Revised Date:  2013-05-27    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  135-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
NESMOS (Neuroscience, Mental Health, and Sensory Organs) Department, School of Medicine and Psychology, Sapienza University, Sant'Andrea Hospital, Rome,
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MeSH Terms
Antipsychotic Agents / blood*
Chromatography, Liquid / methods*
Limit of Detection
Piperazines / blood*
Quinolones / blood*
Reference Standards
Reproducibility of Results
Solid Phase Extraction
Tandem Mass Spectrometry / methods*
Reg. No./Substance:
0/Antipsychotic Agents; 0/Piperazines; 0/Quinolones; 82VFR53I78/aripiprazole

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