Document Detail

An improved setup for the isolated perfused rat lung.
MedLine Citation:
PMID:  8032099     Owner:  NLM     Status:  MEDLINE    
This article will describe a method for perfusing and ventilating rat lungs which allows assessment of lung mechanics, segmental vascular resistance, gas exchange, and vascular permeability. Isolated perfused rat lungs (IPL) were ventilated by negative pressure ventilation and were perfused at constant pressure with recirculating, albumin-containing Krebs-Henseleit buffer. A newly constructed respirator pump facilitated well-defined ventilation of the lungs and permitted switching between negative and positive pressure ventilation. Using this setup lungs were ventilated with positive pressure during both surgery and measurement of lung weight. Once the lungs were prepared, electronic control circuits ensured stability of the perfusate pH and perfusate pressure. Such preparations were stable for at least 3 hr. Breathing mechanics, for example, tidal volume, pulmonary compliance, and pulmonary resistance, perfusate characteristics, such as pulmonary vascular resistance, pulmonary pre- and postcapillary resistance, perfusate pH, PO2, and PCO2, and the capillary filtration coefficient were determined. All measured parameters were within normal physiological range, and the lungs were biochemically active, and responded to various stimuli.
S Uhlig; L Wollin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of pharmacological and toxicological methods     Volume:  31     ISSN:  1056-8719     ISO Abbreviation:  J Pharmacol Toxicol Methods     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-08-17     Completed Date:  1994-08-17     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9206091     Medline TA:  J Pharmacol Toxicol Methods     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  85-94     Citation Subset:  IM    
Department of Biochemical Pharmacology, University of Konstanz, Federal Republic of Germany.
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MeSH Terms
Lung / physiology*
Perfusion / methods*
Pulmonary Edema / etiology
Rats, Wistar

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