Document Detail


An improved purification approach with high cell viability and low cell loss for cryopreserved hepatocytes.
MedLine Citation:
PMID:  19932092     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A modified purification procedure is described for effectively eliminating dead cells after hepatocyte cryopreservation. Isolated hepatocytes from six pig tissue samples were cryopreserved in liquid nitrogen for 2 weeks. After thawing, we developed a pre-incubation step prior to gradient centrifugation. The hepatocytes were subsequent cultured in suspension overnight (12-16 h), and then dead cells were eliminated by Ficoll 400 purification. The results showed that a high viability (mean of 96%) of cells was obtained, with a low viable cell loss in number (2-5%), by using this modified method.
Authors:
Fan-ying Meng; Zhi-shui Chen; Meng Han; Xin-peng Hu; Ping Zhou
Publication Detail:
Type:  Evaluation Studies; In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-20
Journal Detail:
Title:  Cryobiology     Volume:  60     ISSN:  1090-2392     ISO Abbreviation:  Cryobiology     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-08     Completed Date:  2010-05-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  238-9     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Inc. All rights reserved.
Affiliation:
Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. 963cat@sohu.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Count
Cell Death
Cell Separation / methods*
Cell Survival
Centrifugation, Density Gradient
Cryopreservation / methods*
Ficoll
Hepatocytes* / cytology
Swine
Chemical
Reg. No./Substance:
25702-74-3/Ficoll

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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