| An improved plaque reduction virus neutralization assay for human metapneumovirus. | |
| | |
MedLine Citation:
|
PMID: 17420056 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Virus-neutralizing antibodies against human metapneumovirus (hMPV) have been shown to be important indicators for protection in experimental animal models. An improved plaque reduction virus neutralization assay to detect hMPV-specific neutralizing antibodies was designed using two prototype recombinant hMPV strains expressing green fluorescent protein (GFP). These prototypes represented each of the main antigenic variants of hMPV, because antigenic variability could have implications for vaccine development. The utility of mutations in the F gene resulting in trypsin-independent replication was also tested. Although these mutant hMPV strains could replicate in the absence of trypsin, bigger plaque size was achieved with the addition of trypsin. Insertion of the GFP gene in the genome of hMPV did not affect replication of the virus in vitro. Plaques could be detected by measuring expression of GFP after 5 days by automated scanning. Ferret, hamster, and macaque sera positive for hMPV were compared in a conventional virus neutralization assay and the plaque reduction virus neutralization assay. The results obtained with the two assays were in agreement but the improved plaque reduction virus neutralization assay was faster, more suitable for high throughput testing, and 10-fold more sensitive than the conventional virus neutralization assay. |
| | |
Authors:
|
Miranda de Graaf; Sander Herfst; Eefje J A Schrauwen; Bernadette G van den Hoogen; Albert D M E Osterhaus; Ron A M Fouchier |
Related Documents
:
|
3711866 - Murine pneumonia virus: seroepidemiological evidence of widespread human infection. 4030996 - Assessment of specific virus infectivity and virus neutralization by a progeny virus im... 4318956 - Virus aggregation as the cause of the non-neutralizable persistent fraction. 19701796 - Serological survey of antibodies against bvd virus in camels (camelus dromedarius) in i... 2990666 - Race-related morphologic variations in hepatocellular carcinoma. 12603206 - Parainfluenza virus infection in adult lung transplant recipients: an emergent clinical... |
Publication Detail:
|
Type: Comparative Study; Evaluation Studies; Journal Article Date: 2007-04-08 |
Journal Detail:
|
Title: Journal of virological methods Volume: 143 ISSN: 0166-0934 ISO Abbreviation: J. Virol. Methods Publication Date: 2007 Aug |
Date Detail:
|
Created Date: 2007-07-02 Completed Date: 2007-08-28 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8005839 Medline TA: J Virol Methods Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 169-74 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antibodies, Viral / blood* Cercopithecus aethiops Cricetinae Ferrets Genes, Reporter Green Fluorescent Proteins / analysis, genetics Macaca Metapneumovirus / genetics, immunology*, physiology Neutralization Tests / methods* Plaque Assay Recombination, Genetic Sensitivity and Specificity Serum / immunology Trypsin / metabolism Vero Cells Viral Proteins / genetics Virus Replication / genetics, physiology |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Viral; 0/Viral Proteins; 147336-22-9/Green Fluorescent Proteins; EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Infants' interactions with professional caregivers at 3 and 6 months of age: a longitudinal study.
Next Document: Leading tonically active neurons of the striatum from reward detection to context recognition.