| An improved nitrilase-mediated bioprocess for synthesis of nicotinic acid from 3-cyanopyridine with hyperinduced Nocardia globerula NHB-2. | |
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MedLine Citation:
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PMID: 21104103 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Nitrilase of Nocardia globerula NHB-2 was induced by short-chain aliphatic nitriles (valeronitrile > isobutyronitrile > butyronitrile > propionitrile) and exhibited activity towards aromatic nitriles (benzonitrile > 3-cyanopyridine > 4-cyanopyridine > m-tolunitrile > p-tolunitrile). Hyperinduction of nitrilase (6.67 U mg (DCW) (-1) , 18.7 U mL(-1)) was achieved in short incubation time (30 h, 30°C) through multiple feeding of isobutyronitrile in the growth medium. The nitrilase of this organism exhibits both substrate and product inhibition effects. In a fed batch reaction at 1 L scale using hyperinduced resting cells corresponding to 10 U mL(-1) nitrilase activity (1.5 mg(DCW) mL(-1)), a total of 123.11 g nicotinic acid was produced at a rate of 24 g h(-1) g (DCW) (-1) . |
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Authors:
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Nitya Nand Sharma; Monica Sharma; Tek Chand Bhalla |
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Publication Detail:
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Type: Journal Article Date: 2010-11-21 |
Journal Detail:
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Title: Journal of industrial microbiology & biotechnology Volume: 38 ISSN: 1476-5535 ISO Abbreviation: J. Ind. Microbiol. Biotechnol. Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-08-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9705544 Medline TA: J Ind Microbiol Biotechnol Country: England |
Other Details:
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Languages: eng Pagination: 1235-43 Citation Subset: IM |
Affiliation:
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Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, 171005, India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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