Document Detail


An improved method for staining kinetochores of human chromosomes.
MedLine Citation:
PMID:  19579804     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An improved method, which exhibited simultaneously clearly kinetochores and the nucleolar organizer regions of human chromosomes by pretreating of human metaphase chromosomes with HCl and NaOH, followed by staining with silver nitrate and visualizing using ammoniacal silver, is described in the present communication. It has important role for analysis of kinetochore variation, mechanism of chromosomal non-disjunction as well as identification of fuctional active centromeres.
Authors:
Junlin He; Xueqing Liu; Yubin Ding; Chao Yu; Yaguang Weng; Xuemei Chen; Rufei Gao; Yingxiong Wang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Indian journal of experimental biology     Volume:  47     ISSN:  0019-5189     ISO Abbreviation:  Indian J. Exp. Biol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-07-07     Completed Date:  2009-07-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0233411     Medline TA:  Indian J Exp Biol     Country:  India    
Other Details:
Languages:  eng     Pagination:  376-8     Citation Subset:  IM    
Affiliation:
Department of Genetics, No.1 Yixueyuan Road, Yuzhong Street, Chongqing 400016, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Chromosomes, Human*
Humans
Kinetochores*
Metaphase
Staining and Labeling / methods*

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