Document Detail


An improved method of sequential alcian blue and ammoniacal silver staining of chondroitin sulfate proteoglycan in polyacrylamide gels.
MedLine Citation:
PMID:  2450484     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Partially deglycosylated chondroitin sulfate proteoglycan (CSPG) or peptide fragments obtained from CSPG are not readily detectable in gels by staining with Alcian blue 8GX or ammoniacal silver using the technique of Oakley et al. (B. Oakley, D. Kirsh, and N. Morris (1980) Anal. Biochem. 105, 361). Sequencial staining with both reagents allows visualization of intact CSPG or peptides derived from proteoglycans in polyacrylamide gels at protein concentrations as low as 2 ng/mm2, or glucuronic acid and galactosamine concentrations of 1 ng/mm2 or less. This method is significantly more sensitive and has broader applicability than that described by H. Min and M. Cowman (1986) Anal. Biochem. 155, 275) for staining glycosaminoglycan fragments in polyacrylamide gels.
Authors:
R C Krueger; N B Schwartz
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  167     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1987 Dec 
Date Detail:
Created Date:  1988-04-18     Completed Date:  1988-04-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  295-300     Citation Subset:  IM    
Affiliation:
Kennedy Center for Mental Retardation, Department of Pediatrics, University of Chicago, Illinois 60637.
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MeSH Terms
Descriptor/Qualifier:
Alcian Blue*
Electrophoresis, Polyacrylamide Gel
Indoles*
Proteochondroitin Sulfates / analysis*
Proteoglycans / analysis*
Silver*
Staining and Labeling / methods*
Grant Support
ID/Acronym/Agency:
AM-19622/AM/NIADDK NIH HHS; HD-09402/HD/NICHD NIH HHS; HD-17332/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Indoles; 0/Proteochondroitin Sulfates; 0/Proteoglycans; 0/ammoniacal silver; 12040-44-7/Alcian Blue; 7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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