Document Detail


An improved method for perfusion of the isolated brain of the rat-influence of perfusion conditions and application of analeptic and anticonvulsant drugs.
MedLine Citation:
PMID:  6133039     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for the perfusion of the isolated brain of the rat with synthetic fluorocarbon emulsion is described. The functional states of the brains were investigated using biochemical, biophysical, and histological methods. After 4.5 h of perfusion, all brains were in excellent condition and comparable to the in vivo state. The influence of perfusion conditions on the viability of the brains was studied. Deep hypothermia was well tolerated; at 13 degrees the EEG exhibited a mean frequency of about 8 Hz, while the mean amplitude was about 40% lower than at 28 degrees or 36 degrees. Constant-pressure perfusion did not affect brain function, provided that the pressure amplitude was very slowly decreased. During application of pentylenetetrazole (PTZ) it was found that the threshold concentration of PTZ in the perfusion medium to induce epileptic seizure discharges was 0.13 mg/ml. However, after repeated application of PTZ, the threshold concentration decreased exponentially, indicating a kindling phenomenon. It could be shown that not PTZ as such, but the spike potentials provoked by PTZ, induced the kindling effect. This was inhibited by application of primidone or phenobarbital.
Authors:
M Höller; H Breuer; K Fleischhauer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pharmacological methods     Volume:  9     ISSN:  0160-5402     ISO Abbreviation:  J Pharmacol Methods     Publication Date:  1983 Feb 
Date Detail:
Created Date:  1983-06-10     Completed Date:  1983-06-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7806596     Medline TA:  J Pharmacol Methods     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  19-32     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Animals
Anticonvulsants / pharmacology*
Brain / metabolism*
Central Nervous System Stimulants / pharmacology*
Lactates / metabolism
Lactic Acid
Male
Pentylenetetrazole / pharmacology
Perfusion / methods,  veterinary*
Phenobarbital / pharmacology
Primidone / pharmacology
Pyruvates / metabolism
Pyruvic Acid
Rats / metabolism*
Rats, Inbred Strains / metabolism
Seizures / physiopathology
Chemical
Reg. No./Substance:
0/Anticonvulsants; 0/Central Nervous System Stimulants; 0/Lactates; 0/Pyruvates; 125-33-7/Primidone; 127-17-3/Pyruvic Acid; 50-06-6/Phenobarbital; 50-21-5/Lactic Acid; 54-95-5/Pentylenetetrazole; 56-65-5/Adenosine Triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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