| An improved method for long-term measuring of hemolymph fluctuations of non-essential amino acids, GABA and histamine from freely moving crayfish. | |
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MedLine Citation:
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PMID: 16417925 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The microdialysis method was adapted to obtain long-term hemolymph dialysates from the pericardial cavity of freely moving Procambarus clarkii crayfish, to measure fluctuations of non-essential amino acids, GABA and histamine by high-performance liquid chromatography using off-line fluorometric derivatization. Asp, Ala, Tau, GABA and histamine (HA) reached its maximal concentrations at the daybreak, whereas glutamate (Glu), Gln and Gly peaked at the end of the light period. The minimum and maximal detected amounts for each substance along the 24h cycle were (in microM): 20-300Asp, 100-200Glu, 400-700Gln, 400-600Gly, 100-200Tau, 150-300Ala, 2-10 GABA and 25-250HA. Cocktails containing the relative concentration of each amino acid, GABA and histamine resulted in a hyperpolarization that reduced the spontaneous firing of cultured peptidergic X organ neurons. Glu, GABA and histamine evoked a long-lasting hyperpolarization that suppressed the spontaneous firing, whereas Asp, Gly and Tau evoked a depolarization accompanied with neuronal firing. Finally, neither Ala nor Gln modified the resting membrane potential. |
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Authors:
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Jorge Cebada; Ramón Alvarado-Alvarez; Elizabeth Becerra; Leticia Neri-Bazán; Luisa Rocha; Ubaldo García |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-01-18 |
Journal Detail:
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Title: Journal of neuroscience methods Volume: 153 ISSN: 0165-0270 ISO Abbreviation: J. Neurosci. Methods Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-05-02 Completed Date: 2006-07-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7905558 Medline TA: J Neurosci Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1-7 Citation Subset: IM |
Affiliation:
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Department of Physiology, Biophysics and Neurosciences, CINVESTAV, Mexico. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis* Amino Acids, Essential / analysis Animals Astacoidea / physiology* Behavior, Animal / physiology Cells, Cultured Hemolymph / metabolism* Histamine / analysis* Locomotion / physiology* Microdialysis / instrumentation, methods* Reproducibility of Results Sensitivity and Specificity Time Factors gamma-Aminobutyric Acid / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Amino Acids, Essential; 51-45-6/Histamine; 56-12-2/gamma-Aminobutyric Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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