| An improved method for the electrophoretic separation and fluorometric determination of thiamine and its phosphates in animal tissues. | |
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MedLine Citation:
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PMID: 7390709 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An improved method for electrophoretic separation and fluorometric (or radiometric) determination of thiamine and thiamine mono-, di- and tri-phosphates in animal tissues (liver, small intestine, kidney, heart, brain) is described. The amount of total thiamine handled was 1.5-3.5 microgram. The procedure includes: acid excretion of the compounds from the tissue, deproteinization with TCA, purification of the extract on partially deactivated charcoal, elution with 10% n-propanol in 0.1 N formic acid, lyophilization, electrophoresis on gelatinized cellulose acetate strips, elution of the thiaminic bands with 50% ethanol and finally oxidation to thiochromes and reading of eluate fluorescence in a spectrofluorophotometer. The mean internal recovery was 83.3% and the external one 83.5%. Dephosphorylation of the T phosphates was rather scanty and each thiamine compound was comparably recovered (82.0-85.7%), apparently TTP being the worst and T the best recovered compound. |
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Authors:
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C Patrini; G Rindi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition Volume: 50 ISSN: 0300-9831 ISO Abbreviation: Int J Vitam Nutr Res Publication Date: 1980 |
Date Detail:
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Created Date: 1980-09-23 Completed Date: 1980-09-23 Revised Date: 2007-02-21 |
Medline Journal Info:
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Nlm Unique ID: 1273304 Medline TA: Int J Vitam Nutr Res Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 10-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Chemistry Electrophoresis / methods* Electrophoresis, Cellulose Acetate / methods* Intestine, Small / analysis Kidney / analysis Liver / analysis Male Myocardium / analysis Rats Spectrometry, Fluorescence / methods Thiamine / analysis* Thiamine Monophosphate / analysis Thiamine Pyrophosphate / analysis Thiamine Triphosphate / analysis |
| Chemical | |
Reg. No./Substance:
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154-87-0/Thiamine Pyrophosphate; 3475-65-8/Thiamine Triphosphate; 532-40-1/Thiamine Monophosphate; 59-43-8/Thiamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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