Document Detail


An improved method for the determination of gamma-carboxyglutamic acid in proteins, bone, and urine.
MedLine Citation:
PMID:  6614448     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method of high-performance liquid chromatographic separation of the fluorescence derivative of gamma-carboxyglutamic acid (Gla) is presented. Alkaline hydrolysates of protein samples were reacted with o-phthalaldehyde in the presence of ethanethiol for 2 min, and the fluorescence derivative of gamma-carboxyglutamic acid was resolved from the other amino acids by a short column packed with silica-based anion exchanger under isocratic conditions. By this method, as low as 200 fmol of gamma-carboxyglutamic acid can be quantitatively analyzed within 10 min. The method presented here shortened the analysis time for Gla and was at least 10 times more sensitive than the method we described previously (Anal. Biochem. 117, 259-265, 1981). The application of this method to the formic acid-soluble or insoluble gamma-carboxyglutamic acid-containing proteins in chicken bone and the concomitant increase of gamma-carboxyglutamic acid content in chicken bone with age are reported.
Authors:
M Kuwada; K Katayama
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  131     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1983 May 
Date Detail:
Created Date:  1983-10-21     Completed Date:  1983-10-21     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  173-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
1-Carboxyglutamic Acid / isolation & purification*,  urine
Aging
Animals
Bone and Bones / analysis*
Chickens
Chromatography, High Pressure Liquid
Glutamates / isolation & purification*
Proteins / analysis*
Spectrometry, Fluorescence
Sulfhydryl Compounds
o-Phthalaldehyde
Chemical
Reg. No./Substance:
0/Glutamates; 0/Proteins; 0/Sulfhydryl Compounds; 53445-96-8/1-Carboxyglutamic Acid; 643-79-8/o-Phthalaldehyde; 75-08-1/ethanethiol

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