Document Detail


An improved method for chromosome counting in maize.
MedLine Citation:
PMID:  9408584     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An improved method for counting chromosomes in maize (Zea mays L.) is presented. Application of cold treatment (5C, 24 hr), heat treatment (42 C, 5 min) and a second cold treatment (5C, 24 hr) to root tips before fixation increased the number of condensed and dispersed countable metaphase chromosome figures. Fixed root tips were prepared by the enzymatic maceration-air drying method and preparations were stained with acetic orcein. Under favorable conditions, one preparation with 50-100 countable chromosome figures could be obtained in diploid maize using this method. Conditions affecting the dispersion of the chromosomes are described. This technique is especially useful for determining the somatic chromosome number in triploid and tetraploid maize lines.
Authors:
A Kato
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnic & histochemistry : official publication of the Biological Stain Commission     Volume:  72     ISSN:  1052-0295     ISO Abbreviation:  Biotech Histochem     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1998-01-16     Completed Date:  1998-01-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9107378     Medline TA:  Biotech Histochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  249-52     Citation Subset:  IM    
Affiliation:
Institute of Radiation Breeding, NIAR, MAFF, Ibaraki, Japan. akiokato@irb.affrc.go.jp
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MeSH Terms
Descriptor/Qualifier:
Chromosomes*
Cold Temperature
Diploidy
Heating
Metaphase
Plant Roots / ultrastructure
Zea mays / genetics*,  ultrastructure*

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