| An improved histochemical technique for differentiating adrenaline- and noradrenaline-containing cells. | |
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MedLine Citation:
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PMID: 9580525 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Sevki's histochemical technique allows specific staining of catecholamine-containing cells, yet discrimination between adrenaline-(ADR-) cells and noradrenaline-(NOR-) cells is unreliable, being based on hue differences. METHODS: In this work, histochemical differentiation of ADR- and NOR-cells in rat adrenal medulla was carried out by introducing two modifications to Sevki's technique: 1) employment of aged Giemsa solution, and 2) addition of an alkaline differentiating step. RESULTS: With these changes, ADR-cells stained brown, whereas NOR-cells were deep-green, resulting in a clear-cut differentiation. CONCLUSIONS: The modified technique permits to differentiate ADR- from NOR-cells in the adrenal medulla using only a bright field microscope without any sophisticated equipment. The present procedure is inexpensive and easy to carry out. |
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Authors:
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R Ortíz-Butron; M J Piñón-López; L Juárez-Santiago; R Racotta |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archives of medical research Volume: 29 ISSN: 0188-4409 ISO Abbreviation: Arch. Med. Res. Publication Date: 1998 |
Date Detail:
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Created Date: 1998-05-27 Completed Date: 1998-05-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9312706 Medline TA: Arch Med Res Country: MEXICO |
Other Details:
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Languages: eng Pagination: 75-7 Citation Subset: IM |
Affiliation:
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Departamento de Fisiología, Escuela Nacional de Ciencias Biológícas, Instituto Politécnico Nacional, México, D.F. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenal Medulla
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chemistry*,
cytology Animals Cell Differentiation / physiology Epinephrine / analysis* Histocytochemistry Male Norepinephrine / analysis* Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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51-41-2/Norepinephrine; 51-43-4/Epinephrine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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