Document Detail

An improved high yield method to obtain microsatellite genotypes from red deer antlers up to 200 years old.
MedLine Citation:
PMID:  23347507     Owner:  NLM     Status:  Publisher    
Analysis of DNA from older samples, such as museum specimen, is a promising approach to studying genetics of populations and ecological processes across several generations. Here, we present a method for extracting high quality nuclear DNA for microsatellite analysis from antlers of red deer (Cervus elaphus). The genotyping of individuals was based on nine microsatellite loci. Because the amount of DNA found in antlers was high, we could reduce the amount of sample and chemicals used and shorten the decalcification time in comparison to other methods. Using these methods, we obtained genotypes from antlers up to 200 years old.
G S Hoffmann; E M Griebeler
Related Documents :
94857 - Effect of glucocorticoid hormone on the content and synthesis of nucleic acids in carti...
1748627 - Organ culture as a model for investigating the effects of antimetabolites and nucleosid...
12680247 - Cytotoxic effects and mechanisms of an alteration in the dose and duration of 5-fluorou...
22659117 - Radical induced dna damage by cytotoxic square-planar copper(ii) complexes incorporatin...
24721747 - Methylated trf2 associates with the nuclear matrix and serves as a potential biomarker ...
10871607 - The rad51 family member, rad51l3, is a dna-stimulated atpase that forms a complex with ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-25
Journal Detail:
Title:  Molecular ecology resources     Volume:  -     ISSN:  1755-0998     ISO Abbreviation:  Mol Ecol Resour     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101465604     Medline TA:  Mol Ecol Resour     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 Blackwell Publishing Ltd.
Institute of Zoology, Department of Ecology, Johannes Gutenberg-University of Mainz, D-55099, Mainz, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Skull destruction from intracranial metastasis arising from pulmonary squamous cell carcinoma: a cas...
Next Document:  Hydrogels for lentiviral gene delivery.