| An improved electrotransfection method using square shaped electric impulsions. | |
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MedLine Citation:
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PMID: 2833263 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transfection of DNA into non adherent cells can be achieved by electropermeation. Previously published results, partially successful, were obtained using exponential decaying electric impulsions. However, one limitation of this technique has been the damaging effect of this type of impulsions resulting in poor cell recovery. We report hereby the electropermeation of human lymphoblastoid cell lines using a commercially available electropulsator delivering repeated, short, high voltage, square shaped, electric pulses. The parameters of transfection have been optimized using the "Lucifer Yellow Permeation Assay". With the optimum electric parameters, virtually all the cells were permeated and at least 70% survived the shocking conditions. Both transient expression and permanent integration and expression of DNA was observed. |
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Authors:
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F Presse; A Quillet; L Mir; C Marchiol-Fournigault; J Feunteun; D Fradelizi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 151 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1988 Mar |
Date Detail:
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Created Date: 1988-05-11 Completed Date: 1988-05-11 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 982-90 Citation Subset: IM |
Affiliation:
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Laboratoire d'Immunologie CNRS UA1156/IGR/INSERM Institut Gustave Roussy, Villejuif. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Cell Membrane Permeability Cell Survival DNA / analysis* Electric Stimulation Humans Isoquinolines Lymphocytes / cytology Simian virus 40 / genetics Transfection / methods* beta 2-Microglobulin / genetics |
| Chemical | |
Reg. No./Substance:
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0/Isoquinolines; 0/beta 2-Microglobulin; 77944-88-8/lucifer yellow; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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