Document Detail

An improved and cost-effective cDNA-AFLP method to investigate transcription-derived products when high throughput sequencing is not available.
MedLine Citation:
PMID:  19857529     Owner:  NLM     Status:  MEDLINE    
Although the cost of high throughput sequencing is decreasing, the cost is still often too high for individual projects targeted at, e.g., genome-wide transcription profiling in non-model organisms. Then, a low-cost alternative is cDNA-AFLP, which we have now considerably modified in order to develop a faster and simpler method to identify and analyze genes involved in specific, possibly adaptive characteristics. Particularly, we wanted to exclude repetitive PCR amplifications, extensive cloning and the presence of overlapping transcripts, which all lower the efficiency of the method.
Helena Korpelainen; Kirsi Kostamo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biotechnology     Volume:  145     ISSN:  1873-4863     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-16     Completed Date:  2010-02-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  43-6     Citation Subset:  IM    
Department of Applied Biology, University of Helsinki, Finland.
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MeSH Terms
Algae, Red / genetics
Amplified Fragment Length Polymorphism Analysis / economics,  methods*
DNA Primers
DNA, Complementary / metabolism*
Models, Genetic*
RNA, Algal / analysis
Transcription, Genetic
Reg. No./Substance:
0/DNA Primers; 0/DNA, Complementary; 0/RNA, Algal

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