Document Detail

An improved colorimetric assay for fructosamine.
MedLine Citation:
PMID:  2121089     Owner:  NLM     Status:  MEDLINE    
With the commercial availability of the improved colorimetric assay for fructosamine, we established the fructosamine values for non-diabetic healthy persons. This improved assay incorporates detergent and uricase in a more concentrated nitroblue-tetrazolium reagent base compared to the earlier reagent (Johnson & Baker assay), including a new serum calibrator. An evaluation of the assay showed that fructosamine values obtained with serum, EDTA-plasma and heparin-plasma were comparable to each other. There was no apparent effect on fructosamine by lipids. Within-run precision variations for each specimen type (fructosamine 248-592 mumol/l), determined with a mechanised analyser were less than 3% (0.9-3%). Between-run precision for serum and EDTA-plasma ranged from 2.5% to 5.6%. This fructosamine assay also showed a positive correlation with the Johnson and Baker assay. Based on 48 healthy adults (20 males, 28 females) of mean age 32.9 +/- 9.2 years, the fructosamines ranged from 195 to 304 mumol/l, with a mean 250 +/- 24.3 mumol/l. The 2.5 and 97.5 percentiles were 196.4 mumol/l and 301.8 mumol/l respectively.
W Y Ng; K F Lui; A C Thai
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Annals of the Academy of Medicine, Singapore     Volume:  19     ISSN:  0304-4602     ISO Abbreviation:  Ann. Acad. Med. Singap.     Publication Date:  1990 Jul 
Date Detail:
Created Date:  1990-11-15     Completed Date:  1990-11-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7503289     Medline TA:  Ann Acad Med Singapore     Country:  SINGAPORE    
Other Details:
Languages:  eng     Pagination:  480-3     Citation Subset:  IM    
Department of Medicine, National University of Singapore.
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MeSH Terms
Cholesterol / blood
Colorimetry / methods*
Diabetes Mellitus / blood
Edetic Acid / pharmacology
Heparin / pharmacology
Hexosamines / blood*
Triglycerides / blood
Reg. No./Substance:
0/Hexosamines; 0/Triglycerides; 4429-04-3/Fructosamine; 57-88-5/Cholesterol; 60-00-4/Edetic Acid; 9005-49-6/Heparin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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