| An improved bioprocess for synthesis of acetohydroxamic acid using DTT (dithiothreitol) treated resting cells of Bacillus sp. APB-6. | |
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MedLine Citation:
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PMID: 21489785 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Acyltransferase activity of amidase from Bacillus sp. APB-6 was enhanced (24U) by multiple feedings of N-methylacetamide (70mM) into the production medium. Hyperinduced whole resting cells of Bacillus sp. APB-6 corresponding to 4g/L (dry cell weight), when treated with 10mM DTT (dithiothreitol) resulted in 93% molar conversion of acetamide (300mM) to acetohydroxamic acid in presence of hydroxylamine-HCl (800mM) after 30min at 45°C in a 1L reaction mixture. After lyophilization, a 62g powder containing 34% (wtwt(-1)) acetohydroxamic acid was recovered. This is the first report where DTT has been used to enhance acyltransfer reaction and such high molar conversion (%) of amide to hydroxamates was recorded at 1L scale. |
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Authors:
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Deepak Pandey; Rajendra Singh; Duni Chand |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-11 |
Journal Detail:
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Title: Bioresource technology Volume: - ISSN: 1873-2976 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9889523 Medline TA: Bioresour Technol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171005, India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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