Document Detail


An improved animal model for studying the effect of drugs on myocardial metabolism during ischemia.
MedLine Citation:
PMID:  1208987     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An improved dog model to study the effect of drugs on myocardial metabolism during ischemia is described. A reproducible degree of ischemia could be obtained by partial occlusion of the anterior descending branch of the left coronary artery (LAD), using an inflatable cuff with a micrometer. The possibility of inducing the stenosis twice in the same animal has the advantage that the animal can be used as its own control. The reproducibility of the degree of ischemia was demonstrated by the nonsignificant differences in local venous lactate, inorganic phosphate, and glucose concentrations after the first and second stenosis. The mean pressure difference over the stenosis was used to express the degree of coronary artery narrowing. In this model, one does not have to rely on the collateral circulation in collecting local venous blood. Moreover, it is very likely that this blood is obtained from the most pronounced ischemic area, which was localized with radioactive microspheres. At this degree of stenosis, left ventricular function was not affected too much, as was demonstrated by the slight changes in dP/dt max, and systolic and diastolic aortic pressure after induction of the stenosis. The usefulness of our model to evaluate the activity of drugs is demonstrated by the effect of fentanyl, a potent morphine-like analgesic, on the poststenotic local venous lactate and inorganic phosphate concentrations.
Authors:
A H Jageneau; W van Gerven; R Kruger; H van Belle; R S Reneman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Recent advances in studies on cardiac structure and metabolism     Volume:  10     ISSN:  0363-5872     ISO Abbreviation:  Recent Adv Stud Cardiac Struct Metab     Publication Date:  1975  
Date Detail:
Created Date:  1976-04-01     Completed Date:  1976-04-01     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0325677     Medline TA:  Recent Adv Stud Cardiac Struct Metab     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  331-41     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Coronary Disease / metabolism*
Disease Models, Animal*
Dogs
Female
Fentanyl / pharmacology
Glucose / metabolism
Heart Rate / drug effects
Lactates / metabolism
Male
Pharmacology*
Phosphates / metabolism
Chemical
Reg. No./Substance:
0/Lactates; 0/Phosphates; 437-38-7/Fentanyl; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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