Document Detail


An improved HPLC assay for S-2-(3-aminopropylamino)ethyl phosphorothioate (WR-2721) in plasma.
MedLine Citation:
PMID:  6090354     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An HPLC assay is presented for the detection and quantitation of the radioprotective drug S-2-(3-aminopropylamino)ethyl phosphorothioate (WR-2721, ethiofos) present in plasma. Improved selectivity and a 40-fold increase in sensitivity have been demonstrated over the method previously reported by this laboratory. Using precolumn derivatization with fluorescamine and S-3-(4-aminobutylamino)propyl phosphorothioate (WR-80855, a homolog of WR-2721) as the internal standard, drug levels of 0.05 to 1000 micrograms/mL were determined with excellent precision (CV less than or equal to 5% over the concentration range). An isocratic mobile phase of acetonitrile/ethanol/water (16:7:77) modified with 0.01 M tetrabutylammonium phosphate eluted the drug and the internal standard from the C-18 reverse-phase column in 23 minutes and 26 minutes, respectively. Detector response was linear over the entire range. The assay uses 150 microL of plasma and requires a total chromatography time of about 50 minutes. The method was found suitable for pharmacokinetic studies in a preliminary experiment with a beagle dog in which no interferences due to plasma constituents or drug metabolites were observed.
Authors:
N F Swynnerton; E P McGovern; J A Niño; D J Mangold
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  International journal of radiation oncology, biology, physics     Volume:  10     ISSN:  0360-3016     ISO Abbreviation:  Int. J. Radiat. Oncol. Biol. Phys.     Publication Date:  1984 Sep 
Date Detail:
Created Date:  1984-11-16     Completed Date:  1984-11-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7603616     Medline TA:  Int J Radiat Oncol Biol Phys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1521-4     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amifostine / blood*
Animals
Chromatography, High Pressure Liquid / methods*
Dogs
Male
Organothiophosphorus Compounds / blood*
Radiation-Protective Agents / blood*
Chemical
Reg. No./Substance:
0/Organothiophosphorus Compounds; 0/Radiation-Protective Agents; 20537-88-6/Amifostine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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