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An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase.
MedLine Citation:
PMID:  20506501     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Recently, a codon improved version of the Flpe site specific recombinase, termed Flpo, was reported as having greatly improved performance in mammalian cell applications. However, the degree of improvement could not be estimated because essentially no Flpe activity was observed. Here, we compare Flpe and Flpo accurately in a mammalian cell assay to estimate that Flpo is about five times more active than Flpe and similar to Cre and Dre. Consequently, we generated a Flpo deleter mouse line from the JM8 C57Bl/6 ES cells used in the EUCOMM and KOMP systematic knock-out programs. In breeding experiments, we show that the Flpo deleter delivers complete recombination using alleles that are incompletely recombined by a commonly used Flpe deleter. This indicates that the Flpo deleter is more efficient.
Authors:
Andrea Kranz; Jun Fu; Kristin Duerschke; Stefanie Weidlich; Ronald Naumann; A Francis Stewart; Konstantinos Anastassiadis
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Genesis (New York, N.Y. : 2000)     Volume:  48     ISSN:  1526-968X     ISO Abbreviation:  Genesis     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100931242     Medline TA:  Genesis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  512-20     Citation Subset:  IM    
Copyright Information:
(c) 2010 Wiley-Liss, Inc.
Affiliation:
Genomics, BioInnovationsZentrum, Technische Universität Dresden, Am Tatzberg 47, Dresden, Germany.
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