Document Detail


The importance of short term exposure of C3H 10T1/2 cells to polycyclic hydrocarbons: evidence for hydrocarbon-mediated anticarcinogenic activity.
MedLine Citation:
PMID:  7306958     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Since it has been shown that transformation frequencies (TF) of cultured mammalian cells exposed to polycyclic aromatic hydrocarbons (PAH) reach a maximum with increasing PAH concentration and then decline, we have examined TF in C3H 10T1/2 CL8 (10T1/2) cells as a function of an additional parameter of treatment, length of exposure. A 15-min exposure to either benzo[alpha]pyrene (BP) or 3-methylcholanthrene (3-MC), at concentrations ranging from 0.3 to 10 micrograms/ml, was sufficient to induce transformation suggesting that in 10T1/2 cells, non-induced enzymes of the cytochrome P-450 system are involved in the metabolic activation of PAH. At lower BP concentrations (0.3-1.25 micrograms/ml), TF generally increased with exposure time; at higher BP concentrations (2.5-10 micrograms/ml) maximal TF were achieved with 3 h of exposure. For 3-MC, maximal TF occurred at 0.5-1 h with a concentration of 10 micrograms/ml and at 1-6 h with lower concentrations. Moreover, low TF were obtained after 12-h and 24-h exposures to 10 micrograms/ml BP or 3-MC. These results show that TF depend on both the length of exposure and concentration of PAH. Since both BP and 3-MC are extensively metabolized to polyoxygenated derivatives and conjugates, we suggest that certain metabolites may be anticarcinogenic or antipromoting agents. The identities of such metabolites are yet to be determined.
Authors:
A M Sarrif; J Strobel-Stevens; C Miller; J R Smythies
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cancer letters     Volume:  13     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  1981 Sep 
Date Detail:
Created Date:  1982-02-12     Completed Date:  1982-02-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  291-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Benzopyrenes / metabolism*
Cell Line
Cell Transformation, Neoplastic / drug effects*
Dose-Response Relationship, Drug
Metabolic Detoxication, Drug*
Methylcholanthrene / metabolism*
Mice
Time Factors
Chemical
Reg. No./Substance:
0/Benzopyrenes; 56-49-5/Methylcholanthrene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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