| The importance of carbonic anhydrase II in red blood cells during exposure of chicken embryos to CO2. | |
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MedLine Citation:
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PMID: 20472102 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The importance of carbonic anhydrase (CA) during exposure of chicken embryos to CO(2) during the second half of incubation was investigated. The protein abundance and activity of CAII in erythrocytes was significantly higher in CO(2)-exposed embryos compared to normal conditions. Daily injections of acetazolamide (ATZ), an inhibitor of CA, increased blood P(CO2) and decreased blood pH in both control and CO(2)-incubated embryos. ATZ increased blood bicarbonate concentration in embryos exposed to normal atmosphere and in day-12 embryos exposed to high CO(2). The tendency of an increased blood potassium concentration in ATZ-injected embryos under standard atmospheric conditions might indicate that protons were exchanged with intracellular potassium. However, there was no evidence for such an exchange in CO(2)-incubated ATZ-treated embryos. This study shows for the first time that chicken embryos adapt to CO(2) during the second half of incubation by increasing CAII protein expression and function in red blood cells. This response may serve to "buffer" elevated CO(2) levels. |
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Authors:
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N Everaert; H Willemsen; A Hulikova; H Brown; E Decuypere; P Swietach; V Bruggeman |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-20 |
Journal Detail:
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Title: Respiratory physiology & neurobiology Volume: 172 ISSN: 1878-1519 ISO Abbreviation: Respir Physiol Neurobiol Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-12 Completed Date: 2010-10-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101140022 Medline TA: Respir Physiol Neurobiol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 154-61 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Biosystems, Division Livestock-Nutrition-Quality, KU Leuven, Leuven, Belgium. nadia.everaert@biw.kuleuven.be |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological
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physiology Animals Blotting, Western Body Weight / physiology Carbon Dioxide / pharmacology* Carbonic Anhydrase II / blood* Chick Embryo Embryonic Development Erythrocytes / enzymology* Potassium / blood |
| Grant Support | |
ID/Acronym/Agency:
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//Medical Research Council |
| Chemical | |
Reg. No./Substance:
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124-38-9/Carbon Dioxide; 7440-09-7/Potassium; EC 4.2.1.-/Carbonic Anhydrase II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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