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The impact of two different thaw protocols on outcomes of vitrified cleavage-stage embryos transfer.
MedLine Citation:
PMID:  23250400     Owner:  NLM     Status:  In-Data-Review    
The objective of this study was to evaluate the gestational results obtained with vitrified-thawed human cleavage-stage embryo by two different thaw protocols. Embryo development was observed to cleavage-stage and embryos were cryopreserved by vitrification on day 3 after oocyte retrieval. 51 cycles were thawed using vitrification warming kit with decreasing concentrations of sucrose in 3 dilutions ( 1.0, 0.5 and 0 mol per L respectively) as group 1, 56 cycles were thawed with decreasing concentrations of sucrose in 5 dilutions ( 0.8, 0.6, 0.33, 0.2 and 0 mol per L respectively) as group 2. Embryo survival (> 50 percent intact blastomeres), complete embryo survival (100 percent intact blastomeres), pregnancy and implantation rates were compared, and development rates the day after thawing were also compared. Multivariate analysis showed a significant difference in embryo immediate morphological survival rate, complete survival and clinical pregnancies rate between the two groups respectively (87.0 vs. 98.6 percent, p=0.000; 71.0 vs 82.0 percent embryo subsequent development rates, mean number of transferred embryos was similar between the two groups. (61.4 vs. 61.3 percent, p=0.502; 2.2 +/ 0.5 vs. 2.4 +/- 0.6, p=0.113). In addition, no differences in implantation rate were observed between two groups (17.7 vs. 25.6 percent, P=0.138). No difference in the multiple pregnancy rates was found among the two groups also.
X J Zhang; Y Z Yang; Q Lv; Y Wang; X H Cao; X J Li; M X Liao; C Guo
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cryo letters     Volume:  33     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2012 Nov-Dec
Date Detail:
Created Date:  2012-12-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  England    
Other Details:
Languages:  eng     Pagination:  411-7     Citation Subset:  IM    
Department of assisted reproductive medical center ,Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital,32 Yi Huan Lu Xi Er Duan, Chengdu, Sichuan, China.
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