Document Detail

The impact of neurotrophin-3 on the dorsal root transitional zone following injury.
MedLine Citation:
PMID:  18542093     Owner:  NLM     Status:  MEDLINE    
STUDY DESIGN: Morphological and Stereological assessment of the dorsal root transitional zone (DRTZ) following complete crush injury, using light microscopy (LM) and transmission electron microscopy (TEM).
OBJECTIVES: To assess the effect of exogenous neurotrophin-3 (NT-3) on the response of glial cells and axons to dorsal root damage.
SETTING: Department of Anatomy, University College Cork, Ireland and Department of Physiology, UMDS, University of London, UK.
METHODS: Cervical roots (C6-8) from rats which had undergone dorsal root crush axotomy 1 week earlier, in the presence (n=3) and absence (n=3) of NT-3, were processed for LM and TEM.
RESULTS: Unmyelinated axon number and size was greater in the DRTZ proximal (Central Nervous System; CNS) and distal (Peripheral Nervous System; PNS) compartments of NT-3-treated tissue. NT-3 was associated with a reduced astrocytic response, an increase in the proportion of oligodendrocytic tissue and a possible inhibition or delay of microglial activation. Disrupted-myelin volume in the DRTZ PNS and CNS compartments of treated tissue was lower, than in control tissue. In the PNS compartment, NT-3 treatment increased phagocyte and blood vessel numbers. It decreased myelinating activity, as sheath thickness was significantly lower and may also account for the noted lower Schwann cell and organelle volume in the test group.
CONCLUSIONS: Our observations suggest that NT-3 interacts with non-neuronal tissue to facilitate the regenerative effort of damaged axons. This may be as a consequence of a direct action or indirectly mediated by modulation of non-neuronal responses to injury.
A T Hanna-Mitchell; D O'Leary; M S Mobarak; M S Ramer; S B McMahon; J V Priestley; E N Kozlova; H Aldskogius; P Dockery; J P Fraher
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-10
Journal Detail:
Title:  Spinal cord     Volume:  46     ISSN:  1362-4393     ISO Abbreviation:  Spinal Cord     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-04     Completed Date:  2009-03-05     Revised Date:  2011-06-06    
Medline Journal Info:
Nlm Unique ID:  9609749     Medline TA:  Spinal Cord     Country:  England    
Other Details:
Languages:  eng     Pagination:  804-10     Citation Subset:  IM    
Department of Anatomy/Neuroscience, BioSciences Institute, National University of Ireland, Cork, Ireland.
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MeSH Terms
Axons / drug effects,  metabolism,  pathology
Disease Models, Animal
Gliosis / drug therapy,  pathology,  physiopathology
Microscopy, Electron, Transmission
Myelin Sheath / drug effects,  ultrastructure
Neovascularization, Physiologic / drug effects,  physiology
Nerve Growth Factors / pharmacology,  therapeutic use
Nerve Regeneration / drug effects*,  physiology
Neuroglia / drug effects,  pathology
Neurotrophin 3 / pharmacology*,  therapeutic use
Phagocytosis / drug effects,  physiology
Rats, Sprague-Dawley
Rhizotomy / adverse effects*
Spinal Cord / drug effects*,  pathology
Spinal Nerve Roots / drug effects*,  injuries*,  pathology
Treatment Outcome
Wallerian Degeneration / drug therapy,  pathology,  physiopathology
Reg. No./Substance:
0/Nerve Growth Factors; 0/Neurotrophin 3

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