| The impact of mitochondrial and thermal stress on the bioenergetics and reserve respiratory capacity of fish cell lines. | |
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MedLine Citation:
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PMID: 23113865 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Abstract Various stressors affect the health of wild and cultured fish and can cause metabolic disturbances that first manifest at the cellular level. Here, we sought to further our understanding of cellular metabolism in fish by examining the metabolic responses of cell lines derived from channel catfish Ictalurus puntatus (CCO), white bass Morone chrysops (WBE), and fathead minnow Pimephales promelas (EPC) to both mitochondrial and thermal stressors. Using extracellular flux (EF) technology, we simultaneously measured the oxygen consumption rate (OCR; a measure of mitochondrial function) and extracellular acidification rate (ECAR; a surrogate of glycolysis) in each cell type. We performed a mitochondrial function protocol whereby compounds modulating different components of mitochondrial respiration were sequentially exposed to cells. This provided us with basal and maximal OCR, OCR linked to ATP production, OCR from ion movement across the mitochondrial inner membrane, the reserve capacity, and OCR independent of the electron transport chain. After heat shock, EPC and CCO significantly decreased OCR and all three cell lines modestly increased ECAR. After heat shock, the reserve capacity, the mitochondrial energetic reserve used to cope with stress and increased bioenergetic demand, was unaffected in EPC and CCO and completely abrogated in WBE. These findings provide proof-of-concept experimental data that further highlight the utility of fish cell lines as tools for modeling bioenergetics. Received April 12, 2012; accepted August 5, 2012. |
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Authors:
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Benjamin H Beck; S Adam Fuller |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of aquatic animal health Volume: 24 ISSN: 0899-7659 ISO Abbreviation: J. Aquat. Anim. Health Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-11-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9884881 Medline TA: J Aquat Anim Health Country: United States |
Other Details:
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Languages: eng Pagination: 244-50 Citation Subset: IM |
Affiliation:
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a U.S. Department of Agriculture, Agricultural Research Service , Harry K. Dupree-Stuttgart National Aquaculture Research Center , Post Office Box 1050 , Stuttgart , Arkansas , 72160 , USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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