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The impact of fabrication parameters and substrate stiffness in direct writing of living constructs.
MedLine Citation:
PMID:  22736619     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Biomolecules and living cells can be printed in high-resolution patterns to fabricate living constructs for tissue engineering. To evaluate the impact of processing cells with rapid prototyping (RP) methods we modelled the printing phase of two RP systems that employ biomaterial inks containing living cells: a high-resolution inkjet system (BioJet) and a lower resolution nozzle based contact printing system (PAM(2) ). In the first fabrication method, we reasoned that cell damage occurs principally during drop collision on the printing surface, in the second we hypothesise that shear stresses act on cells during extrusion (within the printing nozzle). The two cases were modelled changing the printing conditions: biomaterial substrate stiffness and volumetric flow rate, respectively in BioJet and PAM(2) . Results show that during inkjet printing impact energies of about 10(-8) J are transmitted to cells, while extrusion energies of the order of 10(-11) J are exerted in direct printing. Viability tests of printed cells can be related to those numerical simulations, suggesting a threshold energy of 10(-9) J to avoid permanent cell damage. To obtain well-defined living constructs, a combination of these methods is proposed for the fabrication of scaffolds with controlled 3D architecture and spatial distribution of biomolecules and cells. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012.
Authors:
Annalisa Tirella; Arti Ahluwalia
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-26
Journal Detail:
Title:  Biotechnology progress     Volume:  -     ISSN:  1520-6033     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 American Institute of Chemical Engineers (AIChE).
Affiliation:
Interdepartmental Research Centre "E.Piaggio", Faculty of Engineering, University of Pisa, Via Diotisalvi 2, 56126 Pisa, Italy; DICCISM, Faculty of Engineering, University of Pisa, Via Diotisalvi 2, 56126 Pisa, Italy. a.tirella@centropiaggio.unipi.it, a.ahluwalia@centropiaggio.unipi.it.
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