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The impact of dehydration rate on the production and cellular location of reactive oxygen species in an aquatic moss.
MedLine Citation:
PMID:  22875812     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background and AimsThe aquatic moss Fontinalis antipyretica requires a slow rate of dehydration to survive a desiccation event. The present work examined whether differences in the dehydration rate resulted in corresponding differences in the production of reactive oxygen species (ROS) and therefore in the amount of cell damage.MethodsIntracellular ROS production by the aquatic moss was assessed with confocal laser microscopy and the ROS-specific chemical probe 2,7-dichlorodihydrofluorescein diacetate. The production of hydrogen peroxide was also quantified and its cellular location was assessed.Key ResultsThe rehydration of slowly dried cells was associated with lower ROS production, thereby reducing the amount of cellular damage and increasing cell survival. A high oxygen consumption burst accompanied the initial stages of rehydration, perhaps due to the burst of ROS production.ConclusionsA slow dehydration rate may induce cell protection mechanisms that serve to limit ROS production and reduce the oxidative burst, decreasing the number of damaged and dead cells due upon rehydration.
Authors:
Ricardo Cruz de Carvalho; Myriam Catalá; Jorge Marques da Silva; Cristina Branquinho; Eva Barreno
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-7
Journal Detail:
Title:  Annals of botany     Volume:  -     ISSN:  1095-8290     ISO Abbreviation:  Ann. Bot.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372347     Medline TA:  Ann Bot     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Universidade de Lisboa, Faculdade de Ciências, Departamento de Biologia Vegetal and Center for Biodiversity, Functional & Integrative Genomics (BioFIG), Campo Grande, Edifício C2, 1749-016 Lisboa, Portugal.
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