Document Detail


The impact of cell source, culture methodology, culture location, and individual donors on gene expression profiles of bone marrow-derived and adipose-derived stromal cells.
MedLine Citation:
PMID:  23145933     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bone marrow (BM) stromal cells (MSCs), also known as mesenchymal stem cells, display a high degree of heterogeneity. To shed light on the causes of this heterogeneity, MSCs were collected from either human BM (n=5) or adipose tissue (AT) (n=5), and expanded using 2 different culture methods: one based on fetal calf serum, and one based on human platelet lysate. After initial expansion, MSCs were frozen, and the vials were transported to 3 different laboratories and grown for 1 passage using the same brand of culture plastic, medium, and supplements. Subsequently, the cells were harvested and assayed for their gene expression profile using the Affymetrix exon microarray platform. Based on gene expression profiles, the most discriminative feature was the anatomical harvesting site, followed by culture methodology. Remarkably, genes in the WNT pathway were expressed at higher levels in BM-derived MSCs than in AT-derived MSCs. Although differences were found between laboratories, cell culture location only slightly affects heterogeneity. Furthermore, individual donors contributed marginally to the observed differences in transcriptomes. Finally, BM-derived MSCs displayed the highest level of similarity, irrespective their culture conditions, when compared to AT-derived cells.
Authors:
Ruurd Torensma; Henk-Jan Prins; Ellen Schrama; Eugène T P Verwiel; Anton C M Martens; Helene Roelofs; Bastiaan J H Jansen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-12-21
Journal Detail:
Title:  Stem cells and development     Volume:  22     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-02     Completed Date:  2013-09-25     Revised Date:  2014-04-01    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1086-96     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / cytology
Adipose Tissue / cytology*,  metabolism
Bone Marrow Cells / cytology*,  metabolism
Cell Culture Techniques*
Cell Differentiation
Cells, Cultured
Gene Expression Profiling*
Humans
Mesenchymal Stromal Cells / cytology*,  metabolism
Wnt Signaling Pathway / genetics
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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