Document Detail

The impact of CRISPR repeat sequence on structures of a Cas6 protein-RNA complex.
MedLine Citation:
PMID:  22238224     Owner:  NLM     Status:  MEDLINE    
The repeat-associated mysterious proteins (RAMPs) comprise the most abundant family of proteins involved in prokaryotic immunity against invading genetic elements conferred by the clustered regularly interspaced short palindromic repeat (CRISPR) system. Cas6 is one of the first characterized RAMP proteins and is a key enzyme required for CRISPR RNA maturation. Despite a strong structural homology with other RAMP proteins that bind hairpin RNA, Cas6 distinctly recognizes single-stranded RNA. Previous structural and biochemical studies show that Cas6 captures the 5' end while cleaving the 3' end of the CRISPR RNA. Here, we describe three structures and complementary biochemical analysis of a noncatalytic Cas6 homolog from Pyrococcus horikoshii bound to CRISPR repeat RNA of different sequences. Our study confirms the specificity of the Cas6 protein for single-stranded RNA and further reveals the importance of the bases at Positions 5-7 in Cas6-RNA interactions. Substitutions of these bases result in structural changes in the protein-RNA complex including its oligomerization state.
Ruiying Wang; Han Zheng; Gan Preamplume; Yaming Shao; Hong Li
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-02-09
Journal Detail:
Title:  Protein science : a publication of the Protein Society     Volume:  21     ISSN:  1469-896X     ISO Abbreviation:  Protein Sci.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-14     Completed Date:  2012-06-15     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  9211750     Medline TA:  Protein Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  405-17     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 The Protein Society.
Department of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida 32306, USA.
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MeSH Terms
Archaeal Proteins / chemistry,  metabolism*
Base Sequence
Inverted Repeat Sequences / physiology*
Models, Molecular
Pyrococcus furiosus / chemistry,  immunology
Pyrococcus horikoshii / chemistry,  immunology
RNA / chemistry,  metabolism*
Reg. No./Substance:
0/Archaeal Proteins; 63231-63-0/RNA

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