Document Detail


An immunoradiometric assay for the measurement of neuropeptide Y in plasma.
MedLine Citation:
PMID:  3841734     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The development of an immunoradiometric assay (IRMA) for the direct measurement of neuropeptide Y (NPY) concentrations in plasma is reported. The assay employs simultaneous addition of 125I-labelled affinity purified sheep anti-(NPY 31-36) immunoglobulin (IgG) and a rabbit anti-NPY serum to 0.25 ml volumes of standard or unknown. After 16 hr incubation at 4 degrees C NPY-bound labelled IgG is precipitated using sheep anti-(rabbit IgG Fc region) IgG coupled to Dynospheres solid phase. Precipitated counts are proportional to the NPY concentration in samples. Using this methodology it is possible to measure basal levels in normal human subjects (range 1-5 fmol/ml). Technical difficulties encountered in raising "site-specific" antisera to NPY during the establishment of this assay are outlined.
Authors:
R Corder; P J Lowry
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Peptides     Volume:  6     ISSN:  0196-9781     ISO Abbreviation:  Peptides     Publication Date:    1985 Nov-Dec
Date Detail:
Created Date:  1986-05-23     Completed Date:  1986-05-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8008690     Medline TA:  Peptides     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1195-200     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adrenal Gland Neoplasms / blood
Chromatography, High Pressure Liquid
Humans
Immune Sera
Immunoglobulin G
Nerve Tissue Proteins / blood*
Neuropeptide Y
Pheochromocytoma / blood
Radioimmunoassay
Chemical
Reg. No./Substance:
0/Immune Sera; 0/Immunoglobulin G; 0/Nerve Tissue Proteins; 0/Neuropeptide Y

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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