Document Detail


An immunohistochemical study of pi class glutathione S-transferase expression in normal human tissue.
MedLine Citation:
PMID:  1977319     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glutathione S-transferases (GSTs), a family of isoenzymes that play an important role in protecting cells from cytotoxic and carcinogenic agents, can be separated by biochemical and immunologic characteristics into three distinct classes named alpha, mu, and pi. Previous studies have indicated that there is marked heterogeneity in the expression of different GST isoenzymes in different normal and malignant tissues. To better understand the regulation of the human pi class glutathione S-transferase isoenzyme (GST-pi), the tissue distribution of this protein wa studied by an immunohistochemical technique using an anti-GST-pi polyclonal antibody in normal paraffin-embedded human tissues. These studies indicate that there is a broad distribution of GST-pi in normal human tissues and establish a precise localization within the different organs studied. GST-pi was expressed predominantly in normal epithelial cells of the urinary, digestive, and respiratory tracts, suggesting a possible role for GST-pi in detoxication and elimination of toxic substances. Previous studies have indicated that GST-pi and the putative drug efflux pump P-glycoprotein are both overexpressed in multidrug-resistant human breast cancer cells and in xenobiotic resistant preneoplastic rat hyperplastic liver nodules. Results from this study indicate that there are also similarities between the normal tissue distribution GST-pi and that previously reported for mammalian P-glycoprotein, particularly in secretory epithelia. This finding suggests that these two gene products, which have been implicated in the development of resistance to cytotoxic drugs, may be coregulated in normal and malignant cells.
Authors:
P Terrier; A J Townsend; J M Coindre; T J Triche; K H Cowan
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The American journal of pathology     Volume:  137     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  1990 Oct 
Date Detail:
Created Date:  1990-11-14     Completed Date:  1990-11-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  845-53     Citation Subset:  AIM; IM    
Affiliation:
Laboratory of Pathology, National Cancer Institute, Bethesda, Maryland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Digestive System / enzymology*
Drug Resistance / genetics
Epithelium / enzymology
Fluorescent Antibody Technique
Gene Expression Regulation, Enzymologic*
Glutathione Transferase / classification,  genetics*
Humans
Isoenzymes / classification,  genetics*
Membrane Glycoproteins / genetics
Mice
Mice, Nude
Muscle, Smooth / enzymology*
Neoplasms, Experimental / enzymology
P-Glycoprotein
Urogenital System / enzymology*
Chemical
Reg. No./Substance:
0/Isoenzymes; 0/Membrane Glycoproteins; 0/P-Glycoprotein; EC 2.5.1.18/Glutathione Transferase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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