Document Detail


An immunohistochemical study of the insulin-, glucagon- and somatostatin-immunoreactive cells in the developing pancreas of the chicken embryo.
MedLine Citation:
PMID:  10798318     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The distributions and relative frequencies of insulin-, glucagon- and somatostatin-immunoreactive cells were studied in dorsal, ventral, third and splenic lobes of developing chicken pancreas during embryonic periods (10 days of incubation to hatching) by immunohistochemical methods. The regions of pancreas were subdivided into three regions: exocrine, light and dark islet. Round, oval and spherical shaped immunoreactive cells were detected in all four lobes. According to developmental stages, the types of lobes and the regions of pancreas showed various distributions and relative frequencies. In the splenic lobes, insulin, glucagon and somatostatin-immunoreactive cells were detected in exocrine, dark islet and light islet from time differentiation of splenic lobes, 13 days of incubation. The insulin- and somatostatin-immunoreactive cells of the third lobes were detected in exocrine and light islets from 10 days of incubation, and in dark islets from 15 and 11 days of incubation respectively. Glucagon-immunoreactive cells were detected in exocrine, dark and light islets from 16, 11 and 19 days of incubation respectively. These immunoreactive cells of the ventral lobes were detected in exocrine and light islets. However, dark islets were not found in this lobe. Insulin-immunoreactive cells were demonstrated from 10 days of incubation in these two regions. Glucagon-immunoreactive cells were detected from 17 days of incubation in exocrine and 16 days of incubation in the light islets. Somatostatin-immunoreactive cells were demonstrated from 11 days of incubation in exocrine and 14 days of incubation in the light islets. In the dorsal lobes, insulin-immunoreactive cells were demonstrated in exocrine, dark and light islets from 12, 14, and 13 days of incubation, respectively. Glucagon- and somatostatin-immunoreactive cells were detected in dark and light islets from 13 and 14 days of incubation, respectively. Glucagon- and somatostatin-immunoreactive cells were demonstrated from 10 and 11 days of incubation in exocrine respectively. Generally, insulin-immunoreactive cells were increased in light islets but decreased in light islets with developmental stages. However, glucagon-immunoreactive cells were decreased in light islets but increased in dark islets. In addition, somatostatin-immunoreactive cells showed the same frequencies in light and dark islets with developmental stages except exocrine which increased with developmental stages.
Authors:
S K Ku; J H Lee; H S Lee
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Tissue & cell     Volume:  32     ISSN:  0040-8166     ISO Abbreviation:  Tissue Cell     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-07-07     Completed Date:  2000-07-07     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0214745     Medline TA:  Tissue Cell     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  58-65     Citation Subset:  IM    
Affiliation:
Pharmacology & Toxicology Laboratory, Dong-Wha Pharmaceutical Industrial Co. Anyang, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chick Embryo
Glucagon / analysis
Immunohistochemistry
Insulin / analysis
Pancreas / chemistry,  embryology*
Pancreatic Hormones / analysis*
Somatostatin / analysis
Chemical
Reg. No./Substance:
0/Pancreatic Hormones; 11061-68-0/Insulin; 51110-01-1/Somatostatin; 9007-92-5/Glucagon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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