Document Detail


The immunohistochemical evaluation of VEGF in placenta biopsies of pregnancies complicated by preeclampsia.
MedLine Citation:
PMID:  17710429     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The study was designed to determine the protein levels of vascular endothelial growth factor (VEGF) in the placenta biopsies of patients with preeclampsia and compare with normal controls. DESIGN: Prospective cohort study. METHODS: The placental biopsies were obtained from ten patients with preeclampsia and ten patients of control group at the time of delivery. Avidin-biotin-peroxidase immunohistochemistry was then performed to identify levels of VEGF protein within the tissue and a semi-quantitative method was devised to score the amount of staining present in the sample. Two histopathologists who were blinded to the groups were asked to score each sample for the intensity of staining and the number of cells stained in a randomly selected per high-power fields of each sample. The resulting "H-score" was computed as a product of intensity and percent of cells stained. RESULTS: The VEGF expression was significantly higher in placenta biopsies of preeclamptic patients compared to that of controls (271.2 +/- 22.65 vs. 201.9 +/- 12.33, P = 0.000). CONCLUSION: Immunostaining of VEGF is significantly higher in placenta biopsies of patients with preeclampsia.
Authors:
Fuat Akercan; Teksin Cirpan; M Cosan Terek; H Tayfun Ozcakir; Gulsen Giray; Sermet Sagol; Nedim Karadadas
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Publication Detail:
Type:  Journal Article     Date:  2007-08-21
Journal Detail:
Title:  Archives of gynecology and obstetrics     Volume:  277     ISSN:  0932-0067     ISO Abbreviation:  Arch. Gynecol. Obstet.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2007-12-20     Completed Date:  2008-08-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8710213     Medline TA:  Arch Gynecol Obstet     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  109-14     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Ege University Faculty of Medicine, Bornova, Izmir, 35100, Turkey.
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MeSH Terms
Descriptor/Qualifier:
Adult
Biopsy
Case-Control Studies
Female
Humans
Immunohistochemistry
Microscopy
Placenta / metabolism*,  pathology*
Pre-Eclampsia / metabolism*
Pregnancy
Prospective Studies
Vascular Endothelial Growth Factor A / metabolism*
Chemical
Reg. No./Substance:
0/Vascular Endothelial Growth Factor A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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