Document Detail

An immunocytochemical study of regeneration of gastric epithelia in rat experimental ulcers.
MedLine Citation:
PMID:  16378232     Owner:  NLM     Status:  MEDLINE    
We experimentally observed the process of regeneration of gastric mucosal cells in ulcers induced by surgical removal of the fundic mucosa of rats. The techniques utilized were immunocytochemistry, laser confocal microscopy, and transmission electron microscopy (TEM). Routine TEM and PAS reaction were used for parietal cells, chief cells, and mucous cells. As markers of parietal cells, H+-K+ ATPase, Na+-K+ ATPase, carbonic anhydrase, and aquaporin 4 (AQP4) were used, and for chief cells pepsinogen was used. Healing process of mucosal defect was as follows. On day 2 after the operation, the single-layered regenerating epithelium (RE) originating from the marginal epithelium of the ulcer extended over the granulation tissue of the ulcer base towards the center. Regenerating glands (RGs) appeared in the ulcer margin. The cells appearing first in the RE were undifferentiated cells that had a high nucleus: cytoplasm ratio and abundant free ribosomes. On day 5, the ulcer was almost filled with RGs. Most cells stained positive for PAS reaction. A few immature parietal cells stained weakly with H+-K+ ATPase, Na+-K+ ATPase, carbonic anhydrase, and aquaporin-4 antibodies, and a few immature chief cells stained weakly with pepsinogen antibody were also observed on day 5. On day 10, the ulcer was filled with RGs. The RGs in the periphery of the ulcer stained positive for markers of mature parietal cells and chief cells, whereas the center of the ulcer was composed of immature parietal cells and chief cells. By day 25, the mucosal defect was filled with normal gastric glands formed by maturation of the RGs. The undifferentiated cells that first appeared in the ulcer margin seem to differentiate to special functioning cells of the stomach 5-10 days after ulcer formation.
Takanori Matsuoka; Michiya Kobayashi; Takeki Sugimoto; Keijiro Araki
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Medical molecular morphology     Volume:  38     ISSN:  1860-1480     ISO Abbreviation:  Med Mol Morphol     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-26     Completed Date:  2006-02-01     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  101239023     Medline TA:  Med Mol Morphol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  233-42     Citation Subset:  IM    
Department of Tumor Surgery, Kochi Medical School, Oko-cho, Nankoku, Kochi, 783-8505, Japan.
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MeSH Terms
Cell Differentiation
Disease Models, Animal
Epithelium / pathology*,  physiology*
Parietal Cells, Gastric / cytology,  pathology,  ultrastructure
Rats, Wistar
Regeneration / physiology*
Stomach / cytology*,  pathology*,  ultrastructure
Stomach Ulcer / chemically induced,  pathology*

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