| An immunoblotting procedure comprising O = 9,12 and H = d antigens as an alternative to the Widal agglutination assay. | |
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MedLine Citation:
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PMID: 10193329 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: To compare the established Widal agglutination assay with an immunoblotting procedure. METHODS: 110 sera were used to compare the established Widal agglutination assay with an immunoblotting procedure incorporating lipopolysaccharide (LPS) (O = 9,12) and flagellar (H = d) antigens. RESULTS: Antibodies to the LPS antigens were detected in 18 sera by the Widal assay and in 37 by immunoblotting. Antibodies to the flagellar antigens were detected in 27 sera by Widal assay and in 25 by immunoblotting. CONCLUSIONS: An immunoblotting procedure incorporating O = 9,12 LPS and H = d flagellar antigens was rapid and more sensitive than the established Widal agglutination assay for providing evidence of infection with S typhi. |
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Authors:
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H Chart; L R Ward; B Rowe |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of clinical pathology Volume: 51 ISSN: 0021-9746 ISO Abbreviation: J. Clin. Pathol. Publication Date: 1998 Nov |
Date Detail:
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Created Date: 1999-04-22 Completed Date: 1999-04-22 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0376601 Medline TA: J Clin Pathol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 854-6 Citation Subset: AIM; IM |
Affiliation:
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Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK. hchart@phls.co.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Agglutination Tests Antibodies, Bacterial / blood Antigens, Bacterial / immunology* Electrophoresis, Polyacrylamide Gel Flagella / immunology Humans Immunoblotting / methods* Lipopolysaccharides / immunology Salmonella typhi / immunology* Typhoid Fever / diagnosis* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Lipopolysaccharides |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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