Document Detail


An immunoblotting procedure comprising O = 9,12 and H = d antigens as an alternative to the Widal agglutination assay.
MedLine Citation:
PMID:  10193329     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: To compare the established Widal agglutination assay with an immunoblotting procedure. METHODS: 110 sera were used to compare the established Widal agglutination assay with an immunoblotting procedure incorporating lipopolysaccharide (LPS) (O = 9,12) and flagellar (H = d) antigens. RESULTS: Antibodies to the LPS antigens were detected in 18 sera by the Widal assay and in 37 by immunoblotting. Antibodies to the flagellar antigens were detected in 27 sera by Widal assay and in 25 by immunoblotting. CONCLUSIONS: An immunoblotting procedure incorporating O = 9,12 LPS and H = d flagellar antigens was rapid and more sensitive than the established Widal agglutination assay for providing evidence of infection with S typhi.
Authors:
H Chart; L R Ward; B Rowe
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of clinical pathology     Volume:  51     ISSN:  0021-9746     ISO Abbreviation:  J. Clin. Pathol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-04-22     Completed Date:  1999-04-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0376601     Medline TA:  J Clin Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  854-6     Citation Subset:  AIM; IM    
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK. hchart@phls.co.uk
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MeSH Terms
Descriptor/Qualifier:
Agglutination Tests
Antibodies, Bacterial / blood
Antigens, Bacterial / immunology*
Electrophoresis, Polyacrylamide Gel
Flagella / immunology
Humans
Immunoblotting / methods*
Lipopolysaccharides / immunology
Salmonella typhi / immunology*
Typhoid Fever / diagnosis*
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Lipopolysaccharides
Comments/Corrections

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