Document Detail

An immunoblotting analysis of cross-reactivity between melon, and plantago and grass pollens.
MedLine Citation:
PMID:  9015782     Owner:  NLM     Status:  MEDLINE    
It is known that most patients with type I allergy to pollens also suffer intolerance to fruits. Recently, an epidemiological and CAP-inhibition study has shown a new clustering of allergy between melon and Plantago and grass pollens. The aim of the present study was to confirm these results by immunoblotting analysis and inhibition of immunoblotting. Sera from 3 patients with confirmed allergy to melon, and Dactylis glomerata and Plantago lanceolata pollens were used for the in vitro studies. SDS-PAGE and immunoblotting analysis with a pool of sera revealed that several distinct protein bands were shared by the three extracts at 14, 31, and a spectrum between 40 and 70 kDa, approximately. Immunoblotting inhibition experiments, performed with extracts of melon, Plantago and Dactylis, showed that all allergens of melon blotting were almost completely inhibited by grass and Plantago pollen extracts. Inversely, the melon extract was capable of inhibiting IgE-binding to various allergens of Dactylis at high mol mass and partially to the band at 14 kDa. Moreover, the melon almost totally inhibited the IgE-binding capacity to the proteins of Plantago extract. Taken together, the results support the presence of structurally similar allergens in melon, Plantago and grass pollens, and that all allergenic epitopes of the melon are present in these pollens.
J C García Ortiz; P Ventas; P Cosmes; A López-Asunsolo
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of investigational allergology & clinical immunology     Volume:  6     ISSN:  1018-9068     ISO Abbreviation:  J Investig Allergol Clin Immunol     Publication Date:    1996 Nov-Dec
Date Detail:
Created Date:  1997-04-09     Completed Date:  1997-04-09     Revised Date:  2013-07-31    
Medline Journal Info:
Nlm Unique ID:  9107858     Medline TA:  J Investig Allergol Clin Immunol     Country:  SPAIN    
Other Details:
Languages:  eng     Pagination:  378-82     Citation Subset:  IM    
Servicio de Alergia y Asma, Hospital Los Montalvos, Salamanca, Spain.
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MeSH Terms
Cross Reactions
Food Hypersensitivity / immunology
Fruit / immunology*
Plantago / immunology*
Plants, Medicinal*
Poaceae / immunology*
Pollen / immunology*
Rhinitis, Allergic, Seasonal / immunology

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