| The immunobiology of cobalt: demonstration of a potential aetiology for inflammatory pseudotumours after metal-on-metal replacement of the hip. | |
MedLine Citation:
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PMID: 25183586 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Abnormal wear of cobalt-containing metal-on-metal joints is associated with inflammatory pseudotumours. Cobalt ions activate human toll-like receptor 4 (TLR4), which normally responds to bacterial lipopolysaccharide (LPS) in sepsis. Activation of TLR4 by LPS increases the expression of chemokines IL-8 and CXCL10, which recruit leukocytes and activated T-cells, respectively. This study was designed to determine whether cobalt induces a similar inflammatory response to LPS by promoting the expression of IL-8 and CXCL10. A human monocytic cell line, derived from acute monocytic leukaemia, was treated with cobalt ions and expression of IL-8 and CXCL10 measured at mRNA and protein levels. Cobalt-treated macrophages showed a 60-fold increase in IL-8 mRNA, and an eightfold increase in production of the mature chemokine (both p < 0.001); expression of the CXCL10 gene and protein was also significantly increased by cobalt (both p < 0.001). Experiments were also performed in the presence of CLI-095, a TLR4-specific antagonist which abrogated the cobalt-mediated increase in IL-8 and CXCL10 expression. These findings suggest that cobalt ions induce inflammation similar to that observed during sepsis by the simultaneous activation of two TLR4-mediated signalling pathways. These pathways result in increased production of IL-8 and CXCL10, and may be implicated in pseudotumour formation following metal-on-metal replacement. Cite this article: Bone Joint J 2014; 96-B:1172-7. |
Authors:
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H Lawrence; D Deehan; J Holland; J Kirby; A Tyson-Capper |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The bone & joint journal Volume: 96-B ISSN: 2049-4408 ISO Abbreviation: Bone Joint J Publication Date: 2014 Sep |
Date Detail:
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Created Date: 2014-09-03 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101599229 Medline TA: Bone Joint J Country: England |
Other Details:
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Languages: eng Pagination: 1172-7 Citation Subset: AIM; IM |
Copyright Information:
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©2014 The British Editorial Society of Bone & Joint Surgery. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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