The immunobiology of cobalt: demonstration of a potential aetiology for inflammatory pseudotumours after metal-on-metal replacement of the hip. | |
MedLine Citation:
|
PMID: 25183586 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Abnormal wear of cobalt-containing metal-on-metal joints is associated with inflammatory pseudotumours. Cobalt ions activate human toll-like receptor 4 (TLR4), which normally responds to bacterial lipopolysaccharide (LPS) in sepsis. Activation of TLR4 by LPS increases the expression of chemokines IL-8 and CXCL10, which recruit leukocytes and activated T-cells, respectively. This study was designed to determine whether cobalt induces a similar inflammatory response to LPS by promoting the expression of IL-8 and CXCL10. A human monocytic cell line, derived from acute monocytic leukaemia, was treated with cobalt ions and expression of IL-8 and CXCL10 measured at mRNA and protein levels. Cobalt-treated macrophages showed a 60-fold increase in IL-8 mRNA, and an eightfold increase in production of the mature chemokine (both p < 0.001); expression of the CXCL10 gene and protein was also significantly increased by cobalt (both p < 0.001). Experiments were also performed in the presence of CLI-095, a TLR4-specific antagonist which abrogated the cobalt-mediated increase in IL-8 and CXCL10 expression. These findings suggest that cobalt ions induce inflammation similar to that observed during sepsis by the simultaneous activation of two TLR4-mediated signalling pathways. These pathways result in increased production of IL-8 and CXCL10, and may be implicated in pseudotumour formation following metal-on-metal replacement. Cite this article: Bone Joint J 2014; 96-B:1172-7. |
Authors:
|
H Lawrence; D Deehan; J Holland; J Kirby; A Tyson-Capper |
Related Documents
:
|
22988486 - Allelic imbalance in tor1a mrna expression in manifesting and non-manifesting carriers ... 24486656 - Micrornas as potential biomarkers in diseases and toxicology. 24870766 - Stathmin is a marker of progression and poor prognosis in esophageal carcinoma. 23479126 - Decrease of apoptosis markers during adipogenic differentiation of mesenchymal stem cel... 17431666 - A small cysteine-rich extracellular protein, vcrp, is inducible by the sex-inducer of v... 18003856 - Glial cell line-derived neurotrophic growth factor increases motility and survival of c... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The bone & joint journal Volume: 96-B ISSN: 2049-4408 ISO Abbreviation: Bone Joint J Publication Date: 2014 Sep |
Date Detail:
|
Created Date: 2014-09-03 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101599229 Medline TA: Bone Joint J Country: England |
Other Details:
|
Languages: eng Pagination: 1172-7 Citation Subset: AIM; IM |
Copyright Information:
|
©2014 The British Editorial Society of Bone & Joint Surgery. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
MeSH Terms | |
Descriptor/Qualifier:
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evaluation of the magnitude and location of Cam deformity using three dimensional CT analysis.
Next Document: Preventable mortality in geriatric hip fracture inpatients.