| The immortalization of human endometrial cells. | |
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MedLine Citation:
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PMID: 16251735 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The loss of replicative potential with each cell division has been attributed to the progressive shortening of telomeres. This "mitotic clock" occurs because most normal human cells are telomerase-negative. Telomerase is a multicomponent enzyme that prevents loss of telomeric DNA associated with normal cell division. Transfection of cells with vectors expressing the catalytic subunit of human telomerase (hTERT) is often sufficient for immortalization. In this article, we will address this approach in the establishment of immortalized endometrial cells and its value in facilitating in vitro studies. |
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Authors:
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Graciela Krikun; Gil Mor; Charles Lockwood |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Methods in molecular medicine Volume: 121 ISSN: 1543-1894 ISO Abbreviation: Methods Mol. Med. Publication Date: 2006 |
Date Detail:
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Created Date: 2005-10-27 Completed Date: 2005-12-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 101123138 Medline TA: Methods Mol Med Country: United States |
Other Details:
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Languages: eng Pagination: 79-83 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Yale University, New Haven, CT, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Aging Cell Culture Techniques / methods* Cell Separation Cell Survival Cells, Cultured Endometrium / cytology*, growth & development Female Freezing Humans Stromal Cells / cytology Telomerase / genetics, metabolism Transfection |
| Grant Support | |
ID/Acronym/Agency:
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R01 HD33937-06/HD/NICHD NIH HHS; R01 HL70004-01A1/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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