Document Detail


The identification of several prostaglandin moieties in Crassostrea virginica and Mytilus edulis by radioimmunoassay and high performance liquid chromatography.
MedLine Citation:
PMID:  3865234     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The presence of prostaglanin-like compounds was examined by radioimmunoassay and isochratic step-elution high performance liquid chromatography in two commercially important molluscs, the American oyster Crassostrea virginica, and the edible mussel Mytilus edulis. Radioimmunoassays for prostaglandins F2 alpha and E2, and 6-keto F1 alpha (the stable hydrolysis product of prostacyclin) demonstrated mean +/- S.D. levels of 3.19 +/- 2.1, 13.08 +/- 5.4, and 1.5 +/- 0.42 nanograms of prostaglandin equivalents, respectively, in C. virginica extracts. Levels of PGF2 alpha, PGE2, and 6-keto PGF1 alpha were 4.0 +/- 2.2, 33.34 +/- 3.5, and 7.18 +/- 2.3, respectively, in M. edulis samples. Chromatographic analyses of organic extracts of these bivalues demonstrated the presence of one or more moieties possessing retention times similar to, or identical with, standards of PGF2 alpha, PGE2, and 6-keto PGF1 alpha. The immunological and chromatographic data, as well as the polyenoic fatty acid profiles of these species, suggest the presence of immunoactive prostanoids of the 2- and 3-series. These findings are discussed in the context of present knowledge of eicosanoid biochemistry and physiology in marine invertebrates.
Authors:
B A Ruggeri; C A Thoroughgood
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Prostaglandins, leukotrienes, and medicine     Volume:  20     ISSN:  0262-1746     ISO Abbreviation:  Prostaglandins Leukot Med     Publication Date:  1985 Oct 
Date Detail:
Created Date:  1986-01-21     Completed Date:  1986-01-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8206868     Medline TA:  Prostaglandins Leukot Med     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  69-77     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bivalvia / analysis*
Chromatography, High Pressure Liquid / methods
Ostreidae / analysis*
Prostaglandins / analysis*
Radioimmunoassay / methods
Species Specificity
Tritium
Chemical
Reg. No./Substance:
0/Prostaglandins; 10028-17-8/Tritium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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