Document Detail


The identification of a second cell cycle control on the HO promoter in yeast: cell cycle regulation of SW15 nuclear entry.
MedLine Citation:
PMID:  2167175     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
HO encodes a site-specific endonuclease that initiates mating type switching in S. cerevisiae. It is expressed only transiently during the cell cycle of mother cells, as they undergo Start, but not in daughter cells. Since SWI5 appears to be the only HO transcription factor missing when daughter cells undergo Start, we were interested in the intracellular distribution of SWI5 at cell division. We discovered that SWI5 is found equally concentrated in the nuclei of both mother and daughter cells at the end of anaphase, suggesting that its subsequent fate must somehow differ. Prior to the end of anaphase, SWI5 accumulates in the cytoplasm and only moves into the nucleus when cells enter G1. A version of the HO promoter that has lost its dependence on Start is nevertheless still strongly cell cycle regulated and is activated when SWI5 moves into the nucleus.
Authors:
K Nasmyth; G Adolf; D Lydall; A Seddon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell     Volume:  62     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1990-09-27     Completed Date:  1990-09-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  631-47     Citation Subset:  IM    
Affiliation:
Research Institute of Molecular Pathology, Vienna, Austria.
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MeSH Terms
Descriptor/Qualifier:
Cell Compartmentation
Cell Cycle*
Cell Nucleus / metabolism*
Endonucleases / genetics*
Fluorescent Antibody Technique
Gene Expression Regulation, Fungal*
Genes, Fungal*
Promoter Regions, Genetic
Recombinant Proteins
Saccharomyces cerevisiae / genetics*
Transcription Factors / metabolism*
Tubulin / metabolism
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 0/Transcription Factors; 0/Tubulin; EC 3.1.-/Endonucleases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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