| The identification of Wos2, a p23 homologue that interacts with Wee1 and Cdc2 in the mitotic control of fission yeasts. | |
| | |
MedLine Citation:
|
PMID: 10581266 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The Wee1 kinase inhibits entry into mitosis by phosphorylation of the Cdc2 kinase. Searching for multicopy suppressors that abolish this inhibition in the fission yeast, we have identified a novel gene, here named wos2, encoding a protein with significant homology to human p23, an Hsp90-associated cochaperone. The deletion mutant has a modest phenotype, being heat-shock sensitive. Using antibodies raised against bacterially produced protein, we determined that Wos2 is very abundant, ubiquitously distributed in the yeast cell, and its expression dropped drastically as cells entered into early stationary phase, indicating that its function is associated with cell proliferation. In proliferating cells, the amount of Wos2 protein was not subjected to cell cycle regulation. However, in vitro assays demonstrated that this Hsp90 cochaperone is potentially regulated by phosphorylation. In addition to suppressing Wee1 activity, overproduction of Wos2 displayed synthetic lethality with Cdc2 mutant proteins, indicating that this Hsp90 cochaperone functionally interacts with Cdc2. The level of Cdc2 protein and its associated H1 kinase activity under synthetic lethal conditions suggested a regulatory role for this Wos2-Cdc2 interaction. Hsp90 complexes are required for CDK regulation; the synergy found between the excess of Wos2 and a deficiency in Hsp90 activity suggests that Wos2 could specifically interfere with the Hsp90-dependent regulation of Cdc2. In vitro analysis indicated that the above genetic interactions could take place by physical association of Wos2 with the single CDK complex of the fission yeast. Expression of the budding yeast p23 protein (encoded by the SBA1 gene) in the fission yeast indicated that Wos2 and Sba1 are functionally exchangeable and therefore that properties described here for Wos2 could be of wide significance in understanding the biological function of cochaperone p23 in eukaryotic cells. |
| | |
Authors:
|
M J Muñoz; E R Bejarano; R R Daga; J Jimenez |
Related Documents
:
|
9543386 - C-tak1 protein kinase phosphorylates human cdc25c on serine 216 and promotes 14-3-3 pro... 7841526 - Cell cycle regulation of the p34cdc2 inhibitory kinases. 15157496 - Rum1: a cdk inhibitor regulating g1 progression in fission yeast. 16046476 - Inhibitory phosphorylation of a mitotic cyclin-dependent kinase regulates the morphogen... 15557816 - Involvement of sox-4 in the cytochrome c-dependent aif-independent apoptotic pathway in... 10889466 - Endothelin-1 induces vascular endothelial growth factor synthesis in osteoblasts: invol... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Genetics Volume: 153 ISSN: 0016-6731 ISO Abbreviation: Genetics Publication Date: 1999 Dec |
Date Detail:
|
Created Date: 2000-02-08 Completed Date: 2000-02-08 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0374636 Medline TA: Genetics Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1561-72 Citation Subset: IM |
Affiliation:
|
Departamento de Genética, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, Spain. jimmar@uma.es |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence DNA Primers Fungal Proteins / chemistry, genetics*, metabolism Gene Expression Regulation, Fungal HSP90 Heat-Shock Proteins / antagonists & inhibitors Humans Mitosis* Molecular Sequence Data Schizosaccharomyces / cytology*, metabolism Sequence Homology, Amino Acid |
| Chemical | |
Reg. No./Substance:
|
0/DNA Primers; 0/Fungal Proteins; 0/HSP90 Heat-Shock Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The role of centromere alignment in meiosis I segregation of homologous chromosomes in Saccharomyces...
Next Document: Transcriptional activation in yeast cells lacking transcription factor IIA.