Document Detail


iTRAQ-based proteomic identification of leucine-rich alpha-2 glycoprotein as a novel inflammatory biomarker in autoimmune diseases.
MedLine Citation:
PMID:  19854709     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To identify a novel serum biomarker of disease activity in inflammatory autoimmune disorders. METHODS: Sera obtained from rheumatoid arthritis (RA) patients before and after anti-TNF therapy were analysed by iTRAQ (isobaric tags for relative and absolute quantitation) quantitative proteomic analysis and further validated by ELISA. RESULTS: Of 326 proteins identified by proteomic analysis, increased serum levels of leucine-rich alpha-2 glycoprotein (LRG) was identified in RA patients before therapy. Serum LRG concentrations were significantly elevated in RA patients compared with healthy controls and decreased after anti-TNF therapy. Furthermore, serum LRG concentrations correlated with disease activity in RA and Crohn's disease (CD). Interestingly, in a subpopulation of patients with active CD and normal C-reactive protein levels, serum LRG concentrations were elevated. CONCLUSIONS: LRG represents a novel serum biomarker for monitoring disease activity during therapy in autoimmune patients, particularly useful in patients with active disease but normal CRP levels.
Authors:
Satoshi Serada; Minoru Fujimoto; Atsushi Ogata; Fumitaka Terabe; Toru Hirano; Hideki Iijima; Shinichiro Shinzaki; Teppei Nishikawa; Tomoharu Ohkawara; Kota Iwahori; Nobuyuki Ohguro; Tadamitsu Kishimoto; Tetsuji Naka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-22
Journal Detail:
Title:  Annals of the rheumatic diseases     Volume:  69     ISSN:  1468-2060     ISO Abbreviation:  Ann. Rheum. Dis.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-18     Completed Date:  2010-04-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372355     Medline TA:  Ann Rheum Dis     Country:  England    
Other Details:
Languages:  eng     Pagination:  770-4     Citation Subset:  IM    
Affiliation:
Laboratory for Immune Signal, National Institute of Biomedical Innovation, 7-6-8 Saito-asagi, Ibaragi, Osaka, Japan.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / therapeutic use
Antirheumatic Agents / therapeutic use
Arthritis, Rheumatoid / diagnosis*,  drug therapy
Autoimmune Diseases / diagnosis*,  drug therapy
Behcet Syndrome / diagnosis
Biological Markers / blood
Blood Proteins / metabolism
C-Reactive Protein / metabolism
Crohn Disease / diagnosis
Enzyme-Linked Immunosorbent Assay
Glycoproteins / blood*
Humans
Proteomics / methods
Reproducibility of Results
Tumor Necrosis Factor-alpha / antagonists & inhibitors
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antirheumatic Agents; 0/Biological Markers; 0/Blood Proteins; 0/Glycoproteins; 0/LRG1 protein, human; 0/Tumor Necrosis Factor-alpha; 0/infliximab; 9007-41-4/C-Reactive Protein

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