Document Detail

iTRAQ analysis indicates that exercise normalizes altered expression of proteins in the ventral hippocampus of rats subjected to maternal separation.
MedLine Citation:
PMID:  22080486     Owner:  NLM     Status:  Publisher    
Many studies have reported on the detrimental effects of early life adversity and the beneficial effects of exercise on brain function. However, the molecular mechanisms that underpin these various effects remain poorly understood. The advent of advanced proteomic analysis techniques has enabled simultaneous measurement of protein expression in a wide range of biological systems. We therefore used iTRAQ proteomic analysis of protein expression to determine whether exercise counteracts the detrimental effects of early life adversity in the form of maternal separation (MS) on protein expression in the brain. Rat pups were subjected to MS from postnatal day 2-14 for 3h/day, or normally reared (NR). At 40 days of age, half of the rats in each group (MS, NR) were allowed to exercise voluntarily (access to a running wheel) for 6 weeks and the remainder kept as sedentary controls. At 83 days of age, rats were killed and the ventral hippocampus dissected for quantitative proteomic (iTRAQ) analysis. iTRAQ proteomic analysis identified several proteins that had been altered by MS (including proteins involved in neuronal structure, metabolism, signaling, anti-oxidative stress and neurotransmission) and that many of these proteins were restored to normal by subsequent exposure to voluntary exercise in adolescence. Our data show that a broad range of proteins play a role in the complex consequences of adversity and exercise.
Willie M U Daniels; Lelanie Marais; Dan J Stein; Vivienne A Russell
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-11
Journal Detail:
Title:  Experimental physiology     Volume:  -     ISSN:  1469-445X     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9002940     Medline TA:  Exp Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
1 University of KwaZulu-Natal;
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