Document Detail


iQ-Check Salmonella II: real-time polymerase chain reaction test kit. Performance Tested Method 010803.
MedLine Citation:
PMID:  20166609     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
iQ-Check Salmonella II is a real-time PCR kit for detection of Salmonella in foods. Specific oligonucleotide probes are used to detect target DNA during the amplification, by hybridizing to the amplicons. These probes are linked to a fluorophore, which fluoresces only when hybridized to the target sequence. As part of an Emergency Response Validation due to a massive outbreak and subsequent recall, peanut butter was tested to compare the performance of iQ-Check Salmonella II to the U.S. Food and Drug Administration's Bacteriological Analytical Manual (FDA-BAM) reference method for detection of Salmonella. A single enrichment in buffered peptone water was used for a reduced enrichment time of 21 +/- 1 h over the 48 h reference method. There was no significant difference in the performance of the iQ-Check kit when compared to the FDA-BAM method, as determined by Chi-square analysis. All samples identified as positive by iQ-Check were confirmed by reference method protocol.
Authors:
Wendy F Lauer; Caroline D Sidi; Jean-Philippe Tourniaire
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Publication Detail:
Type:  Journal Article; Validation Studies    
Journal Detail:
Title:  Journal of AOAC International     Volume:  92     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2009 Nov-Dec
Date Detail:
Created Date:  2010-02-19     Completed Date:  2010-03-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1865-70     Citation Subset:  IM    
Affiliation:
Bio-Rad Laboratories, 2000 Alfred Nobel Dr, Hercules, CA 94547, USA. wendy_lauer@bio-rad.com
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MeSH Terms
Descriptor/Qualifier:
DNA, Bacterial / analysis
Genes, Bacterial
Reagent Kits, Diagnostic
Reference Standards
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction / methods*
Salmonella / chemistry*,  genetics*
Salmonella enterica / chemistry,  genetics
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/Reagent Kits, Diagnostic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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