|A hypersensitive response-induced ATPase associated with various cellular activities (AAA) protein from tobacco plants.|
|PMID: 15821994 Owner: NLM Status: MEDLINE|
|The hypersensitive response (HR) is one of the most critical defense systems in higher plants. In order to understand its molecular basis, we have screened tobacco genes that are transcriptionally activated during the early stage of the HR by the differential display method. Among six genes initially identified, one was found encoding a 57 kDa polypeptide with 497 amino acids not showing significant similarity to any reported proteins except for the AAA domain (ATPase associated with various cellular activities) spanning over 230 amino acids. The bacterially expressed protein exhibited ATP hydrolysis activity, and a green fluorescent protein-fusion protein localized in the cytoplasm of onion epidermis cells. The protein was subsequently designated as NtAAA1 (Nicotiana tabacum AAA1). NtAAA1 transcripts were induced 6 h after HR onset not only by TMV but also by incompatible Psuedomonas syringae, indicating that NtAAA1 is under the control of the N-gene with a common role in pathogen responses. Expression of NtAAA1 was induced by jasmonic acid and ethylene, but not by salicylic acid (SA). It also occurred at a high level in SA-deficient tobacco plants upon TMV infection. When NtAAA1 was silenced by the RNAi method, accumulation of transcripts for PR-1a significantly increased during the HR. Treatments with SA induced higher expression of PR-1a and acidic PR-2 in RNAi transgenic plants than in wild-type counterparts. These results suggest that NtAAA1 mitigates the SA signaling pathway, and therefore that NtAAA1 modulates the pathogen response of the host plants by adjusting the HR to an appropriate level.|
|Megumi Sugimoto; Yube Yamaguchi; Kimiyo Nakamura; Yuko Tatsumi; Hiroshi Sano|
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|Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-04-07|
|Title: Plant molecular biology Volume: 56 ISSN: 0167-4412 ISO Abbreviation: Plant Mol. Biol. Publication Date: 2004 Dec|
|Created Date: 2005-04-11 Completed Date: 2005-07-12 Revised Date: 2007-11-15|
Medline Journal Info:
|Nlm Unique ID: 9106343 Medline TA: Plant Mol Biol Country: Netherlands|
|Languages: eng Pagination: 973-85 Citation Subset: IM|
|Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Nara 630-0192, Japan.|
|Data Bank Information|
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|APA/MLA Format Download EndNote Download BibTex|
Amino Acid Sequence
Cyclopentanes / pharmacology
DNA, Complementary / chemistry, genetics, isolation & purification
Ethylenes / pharmacology
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Plant / drug effects
Green Fluorescent Proteins / genetics, metabolism
Molecular Sequence Data
Onions / cytology, metabolism
Plant Diseases / genetics*, microbiology, virology
Plant Proteins / genetics*, metabolism
Plants, Genetically Modified
Pseudomonas syringae / growth & development
Recombinant Fusion Proteins / genetics, metabolism
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Tobacco / genetics*, microbiology, virology
Tobacco Mosaic Virus / growth & development
|0/Cyclopentanes; 0/DNA, Complementary; 0/Ethylenes; 0/Oxylipins; 0/Plant Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; 6894-38-8/jasmonic acid; 74-85-1/ethylene; EC 3.6.1.-/Adenosine Triphosphatases|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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