Document Detail


A hypersensitive response-induced ATPase associated with various cellular activities (AAA) protein from tobacco plants.
MedLine Citation:
PMID:  15821994     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The hypersensitive response (HR) is one of the most critical defense systems in higher plants. In order to understand its molecular basis, we have screened tobacco genes that are transcriptionally activated during the early stage of the HR by the differential display method. Among six genes initially identified, one was found encoding a 57 kDa polypeptide with 497 amino acids not showing significant similarity to any reported proteins except for the AAA domain (ATPase associated with various cellular activities) spanning over 230 amino acids. The bacterially expressed protein exhibited ATP hydrolysis activity, and a green fluorescent protein-fusion protein localized in the cytoplasm of onion epidermis cells. The protein was subsequently designated as NtAAA1 (Nicotiana tabacum AAA1). NtAAA1 transcripts were induced 6 h after HR onset not only by TMV but also by incompatible Psuedomonas syringae, indicating that NtAAA1 is under the control of the N-gene with a common role in pathogen responses. Expression of NtAAA1 was induced by jasmonic acid and ethylene, but not by salicylic acid (SA). It also occurred at a high level in SA-deficient tobacco plants upon TMV infection. When NtAAA1 was silenced by the RNAi method, accumulation of transcripts for PR-1a significantly increased during the HR. Treatments with SA induced higher expression of PR-1a and acidic PR-2 in RNAi transgenic plants than in wild-type counterparts. These results suggest that NtAAA1 mitigates the SA signaling pathway, and therefore that NtAAA1 modulates the pathogen response of the host plants by adjusting the HR to an appropriate level.
Authors:
Megumi Sugimoto; Yube Yamaguchi; Kimiyo Nakamura; Yuko Tatsumi; Hiroshi Sano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-04-07
Journal Detail:
Title:  Plant molecular biology     Volume:  56     ISSN:  0167-4412     ISO Abbreviation:  Plant Mol. Biol.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2005-04-11     Completed Date:  2005-07-12     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9106343     Medline TA:  Plant Mol Biol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  973-85     Citation Subset:  IM    
Affiliation:
Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Nara 630-0192, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB174847
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / genetics*,  metabolism
Amino Acid Sequence
Base Sequence
Cells, Cultured
Cyclopentanes / pharmacology
DNA, Complementary / chemistry,  genetics,  isolation & purification
Ethylenes / pharmacology
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Plant / drug effects
Green Fluorescent Proteins / genetics,  metabolism
Microscopy, Fluorescence
Molecular Sequence Data
Onions / cytology,  metabolism
Oxylipins
Phylogeny
Plant Diseases / genetics*,  microbiology,  virology
Plant Proteins / genetics*,  metabolism
Plants, Genetically Modified
Pseudomonas syringae / growth & development
RNA Interference
Recombinant Fusion Proteins / genetics,  metabolism
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Temperature
Tobacco / genetics*,  microbiology,  virology
Tobacco Mosaic Virus / growth & development
Chemical
Reg. No./Substance:
0/Cyclopentanes; 0/DNA, Complementary; 0/Ethylenes; 0/Oxylipins; 0/Plant Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; 6894-38-8/jasmonic acid; 74-85-1/ethylene; EC 3.6.1.-/Adenosine Triphosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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