| A hypersensitive response-induced ATPase associated with various cellular activities (AAA) protein from tobacco plants. | |
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MedLine Citation:
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PMID: 15821994 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The hypersensitive response (HR) is one of the most critical defense systems in higher plants. In order to understand its molecular basis, we have screened tobacco genes that are transcriptionally activated during the early stage of the HR by the differential display method. Among six genes initially identified, one was found encoding a 57 kDa polypeptide with 497 amino acids not showing significant similarity to any reported proteins except for the AAA domain (ATPase associated with various cellular activities) spanning over 230 amino acids. The bacterially expressed protein exhibited ATP hydrolysis activity, and a green fluorescent protein-fusion protein localized in the cytoplasm of onion epidermis cells. The protein was subsequently designated as NtAAA1 (Nicotiana tabacum AAA1). NtAAA1 transcripts were induced 6 h after HR onset not only by TMV but also by incompatible Psuedomonas syringae, indicating that NtAAA1 is under the control of the N-gene with a common role in pathogen responses. Expression of NtAAA1 was induced by jasmonic acid and ethylene, but not by salicylic acid (SA). It also occurred at a high level in SA-deficient tobacco plants upon TMV infection. When NtAAA1 was silenced by the RNAi method, accumulation of transcripts for PR-1a significantly increased during the HR. Treatments with SA induced higher expression of PR-1a and acidic PR-2 in RNAi transgenic plants than in wild-type counterparts. These results suggest that NtAAA1 mitigates the SA signaling pathway, and therefore that NtAAA1 modulates the pathogen response of the host plants by adjusting the HR to an appropriate level. |
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Authors:
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Megumi Sugimoto; Yube Yamaguchi; Kimiyo Nakamura; Yuko Tatsumi; Hiroshi Sano |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-04-07 |
Journal Detail:
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Title: Plant molecular biology Volume: 56 ISSN: 0167-4412 ISO Abbreviation: Plant Mol. Biol. Publication Date: 2004 Dec |
Date Detail:
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Created Date: 2005-04-11 Completed Date: 2005-07-12 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9106343 Medline TA: Plant Mol Biol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 973-85 Citation Subset: IM |
Affiliation:
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Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Nara 630-0192, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB174847 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphatases
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genetics*,
metabolism Amino Acid Sequence Base Sequence Cells, Cultured Cyclopentanes / pharmacology DNA, Complementary / chemistry, genetics, isolation & purification Ethylenes / pharmacology Gene Expression Regulation, Enzymologic / drug effects Gene Expression Regulation, Plant / drug effects Green Fluorescent Proteins / genetics, metabolism Microscopy, Fluorescence Molecular Sequence Data Onions / cytology, metabolism Oxylipins Phylogeny Plant Diseases / genetics*, microbiology, virology Plant Proteins / genetics*, metabolism Plants, Genetically Modified Pseudomonas syringae / growth & development RNA Interference Recombinant Fusion Proteins / genetics, metabolism Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Temperature Tobacco / genetics*, microbiology, virology Tobacco Mosaic Virus / growth & development |
| Chemical | |
Reg. No./Substance:
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0/Cyclopentanes; 0/DNA, Complementary; 0/Ethylenes; 0/Oxylipins; 0/Plant Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; 6894-38-8/jasmonic acid; 74-85-1/ethylene; EC 3.6.1.-/Adenosine Triphosphatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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