| A hydrogel scaffold that maintains viability and supports differentiation of dental pulp stem cells. | |
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MedLine Citation:
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PMID: 22901827 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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OBJECTIVES: The clinical translation of stem cell-based Regenerative Endodontics demands further development of suitable injectable scaffolds. Puramatrix™ is a defined, self-assembling peptide hydrogel which instantaneously polymerizes under normal physiological conditions. Here, we assessed the compatibility of Puramatrix™ with dental pulp stem cell (DPSC) growth and differentiation. METHODS: DPSC cells were grown in 0.05-0.25% Puramatrix™. Cell viability was measured colorimetrically using the WST-1 assay. Cell morphology was observed in 3D modeling using confocal microscopy. In addition, we used the human tooth slice model with Puramatrix™ to verify DPSC differentiation into odontoblast-like cells, as measured by expression of DSPP and DMP-1. RESULTS: DPSC survived and proliferated in Puramatrix™ for at least three weeks in culture. Confocal microscopy revealed that cells seeded in Puramatrix™ presented morphological features of healthy cells, and some cells exhibited cytoplasmic elongations. Notably, after 21 days in tooth slices containing Puramatrix™, DPSC cells expressed DMP-1 and DSPP, putative markers of odontoblastic differentiation. SIGNIFICANCE: Collectively, these data suggest that self-assembling peptide hydrogels might be useful injectable scaffolds for stem cell-based Regenerative Endodontics. |
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Authors:
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Bruno N Cavalcanti; Benjamin D Zeitlin; Jacques E Nör |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-16 |
Journal Detail:
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Title: Dental materials : official publication of the Academy of Dental Materials Volume: - ISSN: 1879-0097 ISO Abbreviation: Dent Mater Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8508040 Medline TA: Dent Mater Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Cariology, Restorative Sciences, Endodontics, University of Michigan School of Dentistry, Ann Arbor, MI 48109-1078, USA; Department of Restorative Dentistry, São Paulo State University, São José dos Campos, Brazil. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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