Document Detail


A hydrogel scaffold that maintains viability and supports differentiation of dental pulp stem cells.
MedLine Citation:
PMID:  22901827     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: The clinical translation of stem cell-based Regenerative Endodontics demands further development of suitable injectable scaffolds. Puramatrix™ is a defined, self-assembling peptide hydrogel which instantaneously polymerizes under normal physiological conditions. Here, we assessed the compatibility of Puramatrix™ with dental pulp stem cell (DPSC) growth and differentiation.
METHODS: DPSC cells were grown in 0.05-0.25% Puramatrix™. Cell viability was measured colorimetrically using the WST-1 assay. Cell morphology was observed in 3D modeling using confocal microscopy. In addition, we used the human tooth slice model with Puramatrix™ to verify DPSC differentiation into odontoblast-like cells, as measured by expression of DSPP and DMP-1.
RESULTS: DPSC survived and proliferated in Puramatrix™ for at least three weeks in culture. Confocal microscopy revealed that cells seeded in Puramatrix™ presented morphological features of healthy cells, and some cells exhibited cytoplasmic elongations. Notably, after 21 days in tooth slices containing Puramatrix™, DPSC cells expressed DMP-1 and DSPP, putative markers of odontoblastic differentiation.
SIGNIFICANCE: Collectively, these data suggest that self-assembling peptide hydrogels might be useful injectable scaffolds for stem cell-based Regenerative Endodontics.
Authors:
Bruno N Cavalcanti; Benjamin D Zeitlin; Jacques E Nör
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-08-16
Journal Detail:
Title:  Dental materials : official publication of the Academy of Dental Materials     Volume:  29     ISSN:  1879-0097     ISO Abbreviation:  Dent Mater     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-18     Completed Date:  2013-12-10     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  8508040     Medline TA:  Dent Mater     Country:  England    
Other Details:
Languages:  eng     Pagination:  97-102     Citation Subset:  D    
Copyright Information:
Copyright © 2012 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Cell Differentiation / physiology*
Dental Pulp / cytology,  metabolism*
Extracellular Matrix Proteins / metabolism
Humans
Hydrogels / pharmacology*
Materials Testing
Microscopy, Confocal
Odontoblasts / metabolism*
Peptides / chemistry*
Phosphoproteins / metabolism
Real-Time Polymerase Chain Reaction
Sialoglycoproteins / metabolism
Stem Cell Transplantation
Stem Cells / metabolism*
Tissue Scaffolds / chemistry*
Grant Support
ID/Acronym/Agency:
R01 DE021410/DE/NIDCR NIH HHS; R01-DE021410/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/DMP1 protein, human; 0/Extracellular Matrix Proteins; 0/Hydrogels; 0/Peptides; 0/Phosphoproteins; 0/RADA16-I; 0/Sialoglycoproteins; 0/dentin sialophosphoprotein
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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