| The human topoisomerase I damage response plays a role in apoptosis. | |
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MedLine Citation:
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PMID: 15010314 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies have shown that human topoisomerase I cleavage complexes form as a response to various DNA damages in vivo, the so called human topoisomerase I "damage response". It was suggested that this damage response may play a role in DNA repair as well as in apoptosis, but only very few investigations have been done and the significance of the damage response still remains unclear. Here we demonstrate that human topoisomerase I cleavage complexes induced by high doses of UV irradiation are highly stable for up to 48 h. Furthermore, we show that human topoisomerase I cleavage complexes correlate with apoptosis. However, at low UV doses the cleavage complex level was very low and the complexes were repaired. Surprisingly, we found that high levels of stable cleavage complexes were not only found in UV-irradiated cells but also in untreated cells that underwent apoptosis. A possible role of human topoisomerase I in apoptosis is discussed. |
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Authors:
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Kent Søe; Anja Rockstroh; Peter Schache; Frank Grosse |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: DNA repair Volume: 3 ISSN: 1568-7864 ISO Abbreviation: DNA Repair (Amst.) Publication Date: 2004 Apr |
Date Detail:
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Created Date: 2004-03-10 Completed Date: 2004-12-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101139138 Medline TA: DNA Repair (Amst) Country: Netherlands |
Other Details:
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Languages: eng Pagination: 387-93 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Institute of Molecular Biotechnology, Beutenbergstrasse 11, D-07745 Jena, Germany. kent@imb-jena.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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physiology* Blotting, Western DNA Topoisomerases, Type I / physiology* Hela Cells Humans |
| Chemical | |
Reg. No./Substance:
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EC 5.99.1.2/DNA Topoisomerases, Type I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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