Document Detail


Is human placenta proteoglycan remodeling involved in pre-eclampsia?
MedLine Citation:
PMID:  18161024     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Impaired placento-fetal communication is a coherent symptom of exaggerated pre-eclampsia. The impact of the cellular expression of different glycosaminoglycans (GAGs) in this event on the placenta in pre-eclampsia is still obscure. This is the first study aimed at discovering the relationship between structural alterations of different sulfated GAGs at the molecular level and the development of pre-eclampsia in inflicted placenta. Sulfated GAGs were isolated and purified from control and pre-eclampsia placentas. The amount and the molecular weight of GAG in each tissue sample were measured. The polydispersity of the recovered GAG samples were determined by polyacrylamide gel electrophoresis. The disaccharide composition of chondroitin sulfate, dermatan sulfate and heparan sulfate were deduced by chondroitinase and heparinase depolymerization followed by liquid chromatography-mass spectrometry. The in vivo sulfo-modulation of GAGs in pre-eclampsia and control placenta were examined using RT-PCR to determine the transcription levels of different sulfotransferases involved in GAG biosynthesis. Marked differences in GAG sulfation patterns and mRNA level of encoding selected GAG O-sulfotransferases were observed in pre-eclampsia. These data suggest a linkage between pre-eclampsia and the observed alterations in placental GAGs and could provide new insights about the modulating role of GAGs in the development and the severity of placental pre-eclampsia.
Authors:
Mohamad Warda; Fuming Zhang; Moustafa Radwan; Zhenqing Zhang; Nari Kim; Young Nam Kim; Robert J Linhardt; Jin Han
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-12-27
Journal Detail:
Title:  Glycoconjugate journal     Volume:  25     ISSN:  0282-0080     ISO Abbreviation:  Glycoconj. J.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-05-28     Completed Date:  2009-01-07     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  8603310     Medline TA:  Glycoconj J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  441-50     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biological Assay
Disaccharides / analysis,  chemistry
Electrophoresis, Polyacrylamide Gel
Female
Gene Expression Regulation, Enzymologic
Glycosaminoglycans / analysis,  isolation & purification
Humans
Placenta / metabolism*
Pre-Eclampsia / metabolism*
Pregnancy
Proteoglycans / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Sulfotransferases / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
GH 38060/GH/CGH CDC HHS; HL 62244/HL/NHLBI NIH HHS; R01 GM038060/GM/NIGMS NIH HHS; R01 GM038060-19/GM/NIGMS NIH HHS; R01 HL052622/HL/NHLBI NIH HHS; R01 HL052622-09/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Disaccharides; 0/Glycosaminoglycans; 0/Proteoglycans; EC 2.8.2.-/Sulfotransferases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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